四倍体马铃薯SRAP分子遗传连锁图谱的构建

Construction of a genetic linkage map for tetraploid potato based on SRAP markers

为了给后期马铃薯块茎高淀粉、高干物质及产量等重要数量性状基因座(QTL)定位和分子标记辅助育种奠定基础,以四倍体马铃薯YSP-4×MIN-021杂种F1的182个分离单株为作图群体,利用相关序列扩增多态性(SRAP)分子标记进行了遗传图谱构建研究。试验从357对SRAP引物中筛选出适宜引物36对。用这些引物对马铃薯杂种F1的182个分离单株及其亲本的基因组DNA进行PCR扩增得到598个SRAP标记。卡方检验显示偏分离标记数为127个,其中母本连锁群59个,父本连锁群68个,偏分离比率均小于30%,符合遗传作图的要求。用软件JoinMap4.0建立了2张四倍体马铃薯双亲的SRAP遗传图谱。母本YSP-4的连锁群总长度为1572.2cM,标记数目为274个,平均间距为5.74cM,12个连锁群的长度范围为14.03~214.44cM;父本MIN-021的连锁群总长度为1932.23cM,标记数目为324个,平均间距为5.96cM,各连锁群的长度范围为93.65~242.06cM。

This research aimed to lay a foundation for molecular assisted breeding of potato through location of quantitative trait loci(QTL)for important traits such as high starch content,high dry matter content and yield of potato tubers.The mapping population comprised 182 F 1 genotypes from a cross of YSP-4×MIN-021 tetraploid hybrid potato lines.We constructed biparental genetic linkage maps based on sequence related amplified polymorphism(SRAP)molecular makers.A total of 36 out of 357 suitable SRAP primers were selected,the genomic DNA of the 182 F 1 genotypes and their parents was amplified,and a total of 598 SRAP markers was obtained.The chi-square test showed that there were 127 distorted markers,of which 59 were in the maternal linkage group,68 were in the paternal linkage group,and the segregation distortion ratio was less than 30%,which conformed to the requirements for genetic mapping.The two parental SRAP genetic maps of tetraploid potato were established using the software package Join Map 4.0.The maternal map included 274 markers,had a total length of 1572.2 cM with an average interval of 5.74 cM,and the length of the linkage groups ranged from 14.03 cM to 214.44 cM.The paternal map included 324 markers,had a total length of 1932.23 cM with an average interval of 5.96 cM,and the length of the linkage groups ranged from 93.65 cM to 242.06 cM.