大米蛋白质中砷分布规律研究

The Distribution Law of As in Rice Protein

米蛋白在婴幼儿产品和高端产品领域应用具有广阔前景,然而重金属易于在稻米蛋白质中富集增加了米蛋白制品的安全风险。以砷污染大米为研究对象,通过Osborne法连续提取大米中清蛋白、醇溶蛋白、谷蛋白和球蛋白,用SDS-PAGE、MALDI-TOF-MS等方法对蛋白进行表征,并运用红外光谱法,紫外扫描分析研究了各蛋白的光谱吸收特征。利用ICP-MS法测定重金属含量得到不同蛋白结合砷的能力差异,得出砷与清蛋白是优势的结合形态。通过氨基酸组成分析并比对正常大米的氨基酸组成,推测砷可能与蛋白中的脯氨酸结合紧密。

Fhe dominant binding complex in rice.Finally,it was speculated that As probably binds tightly to proline in the protein by analyzing the amino acid composition and comparing with that of normal rice.This study responds to the national quality grain engineering construction and provides a theoretical basis for the development of high-quality rice protein products and rice As reduction technology.