载柚皮苷羟基磷灰石胶原支架对人牙髓干细胞体外增殖及成骨向分化的影响

Naringin in Nanometer Hydroxyapatite Collagen Scaffold Enhanced the Proliferation and Osteoblastic Differentiation of Human Dental Pulp Stem Cells in Vivo

目的:探讨载柚皮苷羟基磷灰石胶原复合支架对人牙髓干细胞体外增殖以及成骨向分化的影响。方法:体外扩增人牙髓干细胞与复合支架联合培养。采用CCK-8法检测人牙髓干细胞在支架上的增殖能力,通过检测碱性磷酸酶活性、茜素红染色和蛋白质印迹法观察支架对细胞体外成骨向分化能力的影响。结果:载柚皮苷羟基磷灰石胶原复合支架能促进牙髓干细胞增殖(P<0.05),并能增加其ALP活性(P<0.05)增强矿化能力,并对成骨相关标记蛋白RUNX-2和BMP-2的表达具有上调作用。结论:载柚皮苷羟基磷灰石胶原支架可提高人牙髓干细胞的增殖能力以及成骨分化的潜能。

Objective:To evaluate the effects of naringin-hydroxyapatite collagen scaffold (Nar-HA/COL) on proliferation and osteoblastic differentiation of human dental pulp stem cells (hDPSCs).Methods:HDPSCs were isolated and cultured on Nar-HA/COL scaffolds.CCK-8 was used to detect the proliferation ability.The osteoblastic differentiation potentials were assessed by alkaline phosphatase assay kit,Alizarin red S histochemical staining,and western Blot.Results:The proliferation ability,ALP activity,mineralization potential,RUNX-2,and BMP-2 proteins expression of hDPSCs were enhanced by 0.5% Nar-HA/COL scaffolds.Conclusion:The results showed Nar-HA/COL scaffolds could enhance the proliferation and osteoblastic differentiation abilities of hDPSCs.