相思子鉴别与含量测定方法的研究

Investigation on identification of content determination of abrine in Abrus precatorius L.

目的:建立相思子粉末显微鉴别、薄层色谱鉴别、主成分相思子碱HPLC法测定方法,提高相思子质量标准。方法:采用显微鉴定法鉴别相思子的粉末显微特征;采用薄层色谱法对相思子的主要成分进行定性鉴别;采用高效液相色谱法测定相思子碱的含量。结果:栅状细胞、子叶细胞、支持细胞特征明显;薄层色谱斑点清晰,专属性强;相思子碱在0.1~4μg范围内线性关系良好(r=0.9999),平均回收率为99.5%,RSD为0.75%(n=6)。结论:本方法操作简便,专属性强,重复性好,可作为相思子的质量控制方法。

Objective : To establish the powder microscopic identification, TLC identification and abrine HPLC determination and to improve the specification of Abrus precatorius L. Methods : The powder characteristics of Abrus precatorius L. was identified by microscopic identification, the main component of Abrus precatorius L. was identified by TLC, and the content of abrine was determined by HPLC. Results : The characteristics of palisade cells, cotyledon cells and supporting cells were obvious. The TLC spots developed were fairly clear and specific. The linear range of albrine fell into 0.1-4 μg ( r =0.999 9). The average recovery was 99.5% with RSD as 0.75%( n= 6). Conclusion : The established methods are simple with high specificity and good repeatability, which can be used for quality control of Abrus precatorius L.