电离辐射对人舌鳞癌CAL-27细胞影响的生物信息学分析

Bioinformatic analysis of ionizing radiation on CAL-27 cells

通过生物信息学方法分析不同辐射源照射对人舌鳞癌CAL-27细胞基因表达谱的影响,为临床筛选口腔肿瘤放射治疗相关分子标志物提供参考.利用X射线和重离子照射CAL-27细胞,运用克隆存活实验及流式细胞术检测细胞存活率及凋亡率;进一步应用高通量测序技术筛选受照射细胞差异表达的miRNAs,并对差异表达基因进行生物学功能及代谢通路富集分析,以探讨其分子损伤机理.结果表明,X射线及重离子照射CAL-27细胞后,细胞存活率分别为88%、71%,细胞凋亡率分别为13.9%、30.4%.通过高通量测序技术检测分析发现,与X射线辐照组相比,重离子辐照组共有33个miRNAs表达差异,其中有12个miRNAs表达上调,21个miRNAs表达下调;通过KEGG(Kyoto encyclopedia of genes and genomes)数据库在表达差异的miRNAs中筛选出了15条相关信号通路,如嗅觉传导通路、细胞因子受体交互通路、丝裂原活化蛋白激酶信号通路等.结果提示,在相同吸收剂量条件下,相比于X射线,重离子辐射对CAL-27细胞miRNA表达谱的影响较大,其变化机理可能与MAPK信号传导通路有关.

To investigate the effects of X-rays and carbon-ion irradiation on CAL-27 cells and identify the differentially expressed microRNA(miRNA),CAL-27 cells were irradiated with X-rays and heavy-ions.Colony survival and apoptosis rate were measured.The differentially expressed miRNAs among different cell groups were obtained by high throughput sequencing technique and analyzed by Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis.After X-ray and heavy-ion irradiation,the survival rate of CAL-27 cells reduced to 88%and 71%,respectively and the rate of apoptosis increased from 1.9%in the control group to 13.9%and 30.4%,respectively.Compared with X-ray irradiated cells,33 miRNAs were changed after heavy-ion irradiation,12 of which were up-regulated and 21 were down-regulated.Fifteen pathways were further filtered out using the KEGG database.Compared with X-ray irradiation,heavy-ion irradiation had a greater effect on the expression of miRNAs in CAL-27 cells at the same dose.The mechanism may be related to the MAPK signal transduction pathways.