羊肚菌发酵液TDS和pH值与生物量的相关性研究

Correlation Between Mycelium Biomass and TDS,pH of Fermentation broth of Morchella spp.

为了快速了解规模化生产羊肚菌液体菌种生物量变化情况,通过测定液体发酵期间菌丝生物量、发酵液中溶解性物质总量(TDS值)和发酵液pH值,考察发酵液TDS值、pH值分别与菌丝生物量之间的相关性。结果表明,在试验期内,随着发酵时间的延长,菌丝生物量先增多后减少,发酵120h为最高值,数据差异极显著;发酵液TDS值先减小后增大,发酵120h为最低值,数据差异极显著;pH值变化范围为6~7,数据差异显著。进一步分析发现,发酵液TDS值和菌丝生物量之间的相关系数r=-0.9054,相关性较强;pH值与菌丝生物量之间的相关系数r=0.5772,相关性较差。因此,在羊肚菌液体发酵期间,可以将发酵液的TDS值作为考察菌丝生物量变化的间接指标,为液体菌种质量标准制定提供了参考依据。

In order to quickly understand the biomass changes of liquid strains of Morchella spp. in large-scale production,the correlation between the mycelium biomass and TDS value,pH value of fermentation broth biomass was investigated by measuring the mycelium biomass,total soluble substances(TDS value) in fermentation broth and fermentation broth pH value during liquid fermentation.The results showed that,during the experimental period,with the extension of fermentation time,the mycelium biomass first increased and then decreased,and reached the maximum value at 120 h,with extremely significant difference in data.The TDS value of fermentation broth first decreased and then increased,and reached the lowest value at 120 h,and the data difference was extremely significant.The pH value ranged from 6 to 7,with significant difference in data.Further analysis showed that the correlation coefficient between the fermentation broth TDS value and mycelium biomass was -0.905 4,indicating a strong correlation between them.The correlation coefficient between pH value and mycelium biomass was 0.577 2,showing a poor correlation.Therefore,during the liquid fermentation of Morchella spp.,the TDS value of the fermentation broth can be used as an indirect indicator to investigate the changes of mycelium biomass,providing reference data for the formulation of the quality standard for liquid strains.