不同氨氮浓度对4株常见藻株生长及酶活性的影响

Effects of Different Concentrations of Ammonia Nitrogen on the Growth and Enzyme Activity of Four Common Algae Strains

高氨氮问题是影响微藻处理养猪沼液的难点.本文以筛选获得的衣藻、葡萄藻、紫球藻和栅藻为研究对象,液体培养下模拟现实沼液废水,分别于培养基中设置50、500和2 000 mg·L^-1的氨氮浓度,探究不同氨氮浓度对微藻生长及藻细胞酶活性的影响.结果表明,不同氨氮浓度下,衣藻和栅藻的生长受到不同程度的抑制,生物量和生物产率均低于正常培养基;50 mg·L^-1氨氮下紫球藻的生物量和生物产率分别为1. 78 g·L^-1和0. 16 g·(L·d)^-1,高于KOCK培养基;500 mg·L^-1氨氮中葡萄藻的生物量和生物产率分别为1. 95 g·L^-1和0. 18 g·(L·d)^-1,高于BG11培养基.各藻种的SOD、POD和CAT均表现为随着氨氮浓度的升高,活性最终呈下降的趋势,丙二醛(MDA)亦然.本研究期望为微藻处理高浓度氨氮沼液提供理论基础.

Treating swine wastewater with a high ammonia nitrogen content with microalgae cultures has proved difficult. In this paper, the strains Chlamydomonas 715,Botryococcus braunii 357,Porphyridium cruentum 806,and Scenedesmus obliquus 417 were tested. Ammonia nitrogen concentrations of 50 mg·L^- 1,500 mg·L ^- 1,and 2 000 mg·L ^- 1 applied to the media according to the concentrations of biogas slurry. This allowed the effect of different concentrations of ammonia nitrogen on the growth and cell enzyme activity of microalgae to be tested. The results showed that the growth of Chlamydomonas 715 and Scenedesmus obliquus 417 was inhibited at different concentrations of ammonia nitrogen,and the biomass and biomass productivities were lower than for the normal media. However,the biomass and biomass productivity of Porphyridium cruentum 806 in 50 mg·L^- 1 ammonia nitrogen were 1. 78 g·L ^-1 and 0. 16 g·(L·d)^- 1,respectively,which were higher than the values obtained using KOCK medium. Furthermore,the biomass and biomass productivity of Botryococcus braunii 357 in 500 mg·L^- 1 ammonia nitrogen were 1. 95 g·L^- 1 and 0. 18 g·(L·d)^- 1, respectively,which were higher than the values obtained using BG11 medium. The SOD,POD,and CAT of all algae species showed a decreasing tendency in response to an increase in the concentration of ammonia nitrogen,as did MDA. These results provide a theoretical basis for the treatment of swine wastewater with high ammonia nitrogen content using microalgae cultures.