脐带间充质干细胞对颅脑创伤患者免疫微环境及血管重建的影响

Effect of umbilical cord mesenchymal stem cells on immune microenvironment and vascular reconstruction in traumatic brain injury

目的探讨脐带间充质干细胞(UCMSCs)对颅脑创伤患者免疫微环境及血管再生重建的影响.方法脑脊液样本分为4组:正常人组、颅脑创伤患者组、颅脑创伤+UCMSCs治疗组和颅脑创伤+常规治疗组,每组6例样本.采用液质联用技术检测脑脊液样本,数据非依赖采集(DIA)技术采集数据,经生物信息学处理筛选出差异蛋白,并对差异蛋白进行基因本体论(GO)分析和京都基因与基因组百科全书(KEGG)分析.结果在脑脊液样本中共鉴定出688种蛋白,并对其进行了可靠的定量分析.经UCMSCs治疗后颅脑创伤患者脑脊液中共有38种差异蛋白,其中上调蛋白20种,下调蛋白18种.GO分析和KEGG分析结果显示,差异蛋白主要为免疫调节功能相关蛋白、血管再生重建相关蛋白以及各类连接蛋白等.结论 UCMSCs治疗颅脑创伤的主要可能作用机制为调节体内免疫微环境的稳定以及促进损伤脑组织的血管再生与重建.

Objective To investigate the effects of umbilical cord mesenchymal stem cells (UCMSCs) on immune microenvironment and angiogenesis in patients with traumatic brain injury.Methods Cerebrospinal fluid(CSF) samples were divided into 4 groups,including normal group (n=6),traumatic brain injury group (n=6),traumatic brain injury+UCMSCs treatment group (n=6) and craniocerebral trauma + conventional treatment group (n=6).The CSF samples were detected by liquid chromatography-mass spectrometry,and data were collected by data independent acquisition (DIA) technology.The differential proteins were screened by bioinformatics processing,and analyzed by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.Results A total of 688 proteins were screened in CSF samples and reliably quantified.There were 38 differential proteins in the CSF of patients with traumatic brain injury after treatment with UCMSCs,including 20 up-regulated proteins and 18 down-regulated proteins.The results of GO analysis and KEGG analysis showed that the differential proteins were mainly immunoregulatory function-related proteins,angiogenesis-related proteins,and various connexins.Conclusions The main possible mechanism of UCMSCs in the treatment of traumatic brain injury is to regulate the stability of the immune microenvironment and to promote the regeneration and reconstruction of damaged brain tissue.