放射治疗对肺癌患者IL-18、sMICA及NK细胞的影响

The effect of radiation therapy on IL-18, sMICA and NK cell of patients with lung cancer

为探讨肺癌患者放射治疗(简称放疗)前及放疗不同阶段NK细胞存活率及IL-18、可溶性MHC-Ⅰ类链相关蛋白A(soluble MHC class Ⅰ-related chain A, sMICA)浓度变化,对肿瘤科收治的30例肺癌放疗患者,通过ELISA检测IL-18、sMICA浓度,应用磁珠分选检测NK细胞存活率。结果显示,sMICA浓度在放疗前最低,而在放疗后,随着放疗剂量的增加逐渐降低(P < 0.05);IL-18浓度随着放疗剂量的增加并无明显变化,且与放疗前无显著差异(P > 0.05);NK细胞活性在放疗后与放疗前无显著差异,且不随剂量增加而明显变化(P > 0.05)。由此,放疗对肺癌患者外周血中NK细胞的存活率和IL-18浓度无明显影响;放疗可降低患者外周血中肿瘤抗原sMICA浓度,表明肿瘤免疫逃逸功能被抑制,放疗可间接增强机体的免疫功能。

In order to investigate the survival rate of NK cells and the changes of IL-18 and soluble MHC class Ⅰ-related chain A (sMICA) concentrations in different stages of radiation therapy (referred to as radiotherapy) for lung cancer patients, we detected IL-18 and sMICA by ELISA and NK cell survival rate by magnetic bead sorting in 30 patients with lung cancer treated by radiotherapy in the Oncology Department. The results showed that the concentration of sMICA before radiotherapy was generally lower than post-radiotherapy, while among the different dosage groups, the concentration of sMICA decreased gradually with the increase of radiation dose (P < 0.05)(2 000, 4 000, 6 000 cGy), while the concentration of IL-18 did not change significantly with the increase of radiotherapy dosage (P > 0.05). NK cell activity did not change significantly with the increase of radiotherapy dosage (P > 0.05). In conclusion, radiation has no significant effect on the survival rate of NK cells in the peripheral blood of lung cancer patients and does not affect the killing effect of NK cells on tumor cells. Radiotherapy can reduce the sMICA concentration in patients' peripheral blood, indicating that the tumor escape is inhibited and thus indirectly enhances the body's immune function.