Erbin在食管鳞状细胞癌中的表达及其与患者预后的关系

Expression of Erbin in esophageal squamous cell carcinoma and its relationship with patient prognosis

目的:探讨Erbin在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中的表达意义及其与患者预后的关系。方法:利用免疫组织化学法检测含有299例食管癌病例的组织芯片中Erbin的表达情况,并分析Erbin的表达与临床病理参数和患者生存时间的关系,采用Cox回归预测各临床病理参数的危险度。此外,借助蛋白印迹和PCR方法检测25例冷冻的食管癌组织和癌旁正常组织中Erbin的蛋白和基因表达。结果:与癌旁正常食管上皮相比,Erbin蛋白和mRNA在ESCC组织中的表达显著增高(55.2%vs. 0,P<0.05),且Erbin的高表达与患者的TNM分期(64.9%vs. 47.3%,P=0.002)和淋巴结转移状态密切相关(65.5%vs.45.0%,P<0.001),Erbin的高表达也与患者的不良预后密切相关(P<0.05)。结论:Erbin在食管鳞状细胞癌中表达增高并与患者的不良预后密切相关,提示Erbin可能是一个重要的肿瘤患者预后的生物标记物。

Objective: To explore the significance of Erbin expression in esophageal squamous cell carcinoma(ESCC) and its relationship with patient prognosis. Methods: Erbin expression in a tissue chip containing samples from 299 cases were examined using immunohistochemistry;in addition, the relationship between Erbin expression and clinical-pathological parameters and patient survival time were also analyzed. Cox regression was used to predict the risk of clinical-pathological parameters. The mRNA and protein expression of Erbin was also determined in 25 cases with paired ESCC and normal tissues through polymerase chain reaction(PCR) and Western blot. Results: The expression of Erbin protein and m RNA in ESCC were significantly higher than those of normal esophageal epithelium adjacent to cancer(55.2% vs. 0, P<0.05). The high expression of Erbin in ESCC was closely related to TNM stage(64.9% vs. 47.3%,P=0.002) and lymph node metastasis(65.5% vs. 45.0%, P<0.001). Moreover, the high expression of Erbin in ESCC was closely related to poor prognosis(P<0.05). Conclusions: Erbin expression was increased in ESCC and was closely related to poor patient prognosis, which suggested that Erbin may be an important biomarker for the prognosis of cancer patients.