基于气生根的雷公藤植株的快速繁殖技术

Tissue Culture and Rapid Propagation of Tripterygium wilfordii Seedlings Based on Air Rooting

以雷公藤幼嫩茎段为外植体材料,研究雷公藤无菌体系的建立,植物生长调节剂以及培养方式对诱导腋芽萌发和茎段快速生根的影响,以期获得雷公藤植株的快速繁殖技术。结果表明:雷公藤外植体的最佳消毒方式是75%酒精30 s+0.1%升汞10 min;腋芽萌发的最佳培养基为MS+1.5 mg·L-1 6-BA+0.2 mg·L-1 NAA,最佳培养条件为温度(23±1)℃,湿度70%±5%,光/暗培养12 h/12 h,腋芽的萌发率为98.3%;生根的最佳培养基为1/2 MS+0.2 mg·L-1 NAA+0.1 mg·L-1 IAA+0.03%活性炭,最佳培养条件为叶片支撑的带芽茎段空气悬浮培养,温度为(23±1)℃,湿度为70%±5%,光/暗培养12 h/12 h,不定根的萌发率达到91.5%。该空气生根的雷公藤体外培养体系方法简单,月增殖系数可达到3.8,可同时快速实现雷公藤植株的增殖培养和生根培养。

To develop new tissue culture and rapid propagation systems of Tripterygium wilfordii Hook.f.,the young shoots were used as explants to induce axillary buds germination.Germinated axillary buds were cut for root inducing and elongation of stems and leaves.The elongated stems were excised into budded stem segments,from which roots were induced in a specific culture medium to achieve rapid plant proliferation.The results showed that the best sterilization effects were achieved when the explants were soaked in 75%alcohol for 30 seconds,washed three times with sterile water,soaked in 0.1% mercury liter solution for 10 minutes.The most effective medium for axillary bud germination was MS medium with 1.5 mg·L-1 6-BA+0.2 mg·L-1 NAA.The efficacy could reach98.3%.The optimum medium for inducing rapid rooting of stem segments was 1/2 MS medium with0.2 mg·L-1 NAA+0.1 mg·L-1 IAA+0.03% activated carbon.The optimal root-inducing condition was air suspension culture with the lower stem ends of ecology at about 1 cm height from the medium at(23±1)℃,and the humidity being 70%±5% by the 12 h/12 hlight/dark culture.The germination efficacy of adventitious roots reached 91.5%.In this study,a tissue culture system for rapid propagation of Tripterygium wilfordii seedlings was established.With this simple method,the monthly proliferation coefficient could reach 3.8.Cutting and transferring budded stem segments to root-inducing medium could realize rapid proliferation of stems and leaves and rooting at the same time.