喷墨全包芯骨支架与脂肪干细胞复合体修复兔桡骨缺损的研究

Repair of rabbit segmental radial defect by a compound of adipose-derived stem cells with the ink-jet all-core structure composite scaffold

目的采用脂肪干细胞(ADSCs)复合喷墨全包芯骨支架修复兔桡骨缺损.方法成功成骨诱导的ADSCs与喷墨全包芯骨支架复合构建细胞支架复合体(A组),ADSCs/聚乳酸-乙醇酸(PLGA)/β磷酸三钙(β-TCP)复合体(B组)、空白缺损(C组)作为对照.采用随机区组设计植入36只兔桡骨1,5 cm缺损部位,术后12、24、48周取材,进行相关分析.结果影像学、大体观察、组织学检测示A组骨缺损完全修复,显著优于B组部分修复,C组没有修复;Lane-Sandhu评分示A组(5.11±0.70)、(8.31±0.48)、(12.98 ±0.60)分,骨缺损修复效果明显好于B组(1.22±0.59)、(4.89 ±0.81)、(6.18 ±0.71)分(t=18.000,P<0.05);B组明显好于C组(0.58±1.00)、(0.58±1.00)、(0.58±1.00)分(t=13.500,P<0.05).结论 ADSCs/喷墨全包芯骨支架复合体可修复兔桡骨1.5 cm缺损.

Objective To repair segmental radial defect by adipose-derived stem cells (ADSCs) combined with a porous wrapped-core structure composite scaffold composed of poly(lactic-co-glycolic acid)/β-tricalciumphosphate (PLGA/β-TCP) skeleton ink-jet wrapped with Type I collagen. Methods 1.5 cm segmental radial defects were created in 36 rabbits, then the combination of ADSCs and ink-jet all-core structure composite scaffold (group A) and the combination of ADSCs and PLGA/β-TCP skeleton (group B) were transplanted into the untreated radial defect as blank control group (group C). 12, 24, 48 weeks after implantation, animals were sacrificed and the constructs were harvested and analyzed. Results Imaging, gross observation and histological examination showed that the bone defect in group A was completely repaired, which was significantly superior to the partial repair in group B and not in group C. In group A, synosteosis was formed between the two ends of the critical-size bone defect 12 weeks postoperation;bone integrity was almost finished and bone marrow started a trend of recanalization 24 weeks postoperation;bone defect repair was completely repaired and bone marrow was recanalized 48 weeks later. As compared with group C at 12, 24, and 48 h [(0.58±1.00),(0.58±1.00) and (0.58±1.00) points], Lane-Sandhu indicated that the new bone forming in group B [(1.22±0.59),(4.89±0.8), and (6.18±0.71) points was significantly increased (t=13.500, P<0.05);As compared with group B, the new bone forming in group A [(5.11±0.70),(8.31±0.48), and (12.98±0.60) points] was significantly increased (t=18.000, P<0.05). Conclusion The combination of ADSCs and ink-jet all-core structure composite scaffold exhibits a favorable osteogenesis, biocompatibility and biodegradability during the repair of autologous segmental radial defect model, which clearly demonstrates promise for the treatment of bone defects through tissue engineering.