非编码RNA HOTAIR和MALAT1在非小细胞肺癌患者血清及支气管肺泡灌洗液中表达水平的临床意义

Clinical significance of non-coding RNA HOTAIR and MALAT1 expression levels in serum and bronchoalveolar lavage fluid of patients with non-small cell lung cancer

目的:探讨非编码RNA HOTAIR和MALAT1在非小细胞肺癌(NSCLC)患者血清及支气管肺泡灌洗液(BALF)中表达水平的临床意义。方法:提取NSCLC组86例患者及对照组30例患者血清和BALF中总RNA,用实时荧光定量RT - PCR检测HOTAIR和MALAT1基因的表达水平并分析其与NSCLC患者临床病理因素的关系,同时分析BALF中HOTAIR和MALAT1的诊断效能。结果: NSCLC组患者血清中HOTAIR表达水平显著高于对照组,但MALAT1的表达水平却低于对照组,差异均有统计学意义( P <0.05)。NSCLC组患者BALF中HOTAIR、MALAT1相对表达水平明显高于对照组,且肿瘤越大,恶性程度越高,其表达水平越高;此外,腺癌患者BALF中MALAT1表达量(1.03±0.26)较鳞癌患者(0.64±0.08)明显增高( P <0.05)。根据约登指数最大法选取BALF中HOTAIR、MALAT1表达量分别为5.16、0.92作为最佳截断值时,诊断肺癌的敏感度分别为73.5%、82.3%,特异度分别为85.4%、87.6%。结论: HOTAIR、MALAT1在NSCLC患者支气管肺泡灌洗液中呈高水平表达,敏感度和特异度较高,对于NSCLC的辅助诊断具有一定的指导价值。

Objective: To investigate the clinical significance of non- coding RNA HOTAIR and MALAT1 expression levels in serum and bronchoalveolar lavage fluid (BALF) of patients with non- small cell lung cancer (NSCLC). Methods: Total RNA in serum and BALF was extracted from 86 NSCLC patients and 30 healthy people, and the expression levels of HOTAIR and MALAT1 genes were detected by real- time fluorescence quantitative RT- PCR, and their correlation with clinicopathological factors in NSCLC patients was analyzed. Meanwhile, the diagnostic efficacy of HOTAIR and MALAT1 in BALF was analyzed. Results: The expression level of HOTAIR in the serum of NSCLC patients was significantly higher than that of the healthy control group, but the expression level of MALAT1 was lower than that of the healthy control group, with statistically significant differences ( P <0.05).The relative expression levels of HOTAIR and MALAT1 in BALF of NSCLC patients were significantly higher than those in the healthy control group. In addition, the relative expression level of MALAT1 in BALF of adenocarcinoma patients (1.03±0.26) was significantly higher than that of squamous cell carcinoma patients (0.64±0.08)( P <0.05).The sensitivity and specificity of HOTAIR in bronchoalveolar lavage fluid of NSCLC patients were 73.5% and 82.3%, respectively (cut off value was 5.16), while the sensitivity and specificity of MALAT1 were 85.4% and 87.6%, respectively (cut off value was 0.92). Conclusion: HOTAIR and MALAT1 are highly expressed in bronchoalveolar lavage fluid of NSCLC patients with high sensitivity and specificity, which has certain guiding value for the auxiliary diagnosis of NSCLC.