食管鳞状细胞癌患者组织中RAB1A基因表达的相关研究及其临床意义

The expression pattern and clinic value of RAB1A in esophageal squamous cell carcinoma

[目的]探讨RAB1A在食管鳞状细胞癌患者中的表达及其临床意义。[方法]采用实时荧光定量PCR检测课题组2013~2015年收集的126例确诊食管鳞状细胞癌患者的癌组织及对应癌旁正常组织中RAB1A的表达量,并根据其表达量高低分为高表达组和低表达组;分析RAB1A表达量与食管鳞状细胞癌患者临床特征、生存期之间的关系;通过过表达RAB1A的表达后检测其对食管鳞状细胞癌细胞系增殖、迁移和侵袭能力的影响。[结果]RAB1A在食管鳞状细胞癌组织中表达量高于对应癌旁正常组织(0.0113±0.0194 vs.0.0071±0.0098,P=0.0123);RAB1A高表达与食管鳞状细胞癌患者家族肿瘤史(P=0.002)、饮酒(P=0.005)、体质指数(P=0.001)、分期(P=0.024)、淋巴结转移(P<0.001)、远端转移(P<0.001)及分化程度(P<0.001)相关;RAB1A高表达与食管鳞状细胞癌患者生存期明显缩短(P<0.001)。RAB1A过表达后促进食管鳞状细胞癌细胞增殖(F=151.9,P<0.001)、transwell迁移(100.4±14.2 vs.51.2±10.6,P=0.009)、划痕实验迁移距离[(655.0±43.9)μmvs.(320.7±115.2)μm,P=0.0003]和transwell侵袭(79.2±13.4 vs.46.2±17.6,P=0.0014)。[结论]RAB1A在食管鳞状细胞癌中高表达,且高表达将缩短食管鳞状细胞癌患者生存期,过表达RAB1A能够促进食管鳞状细胞癌细胞增殖、迁移和侵袭能力。

[Objective]To detect the expression and clinical value of RAB1 Ain patients with esophageal squamous cell carcinoma(ESCC).[Methods]Real-time quantitative PCR(RT-PCR)was used to detect the expression level of RAB1 Ain cancer tissues and adjacent normal tissues of 126 patients diagnosed with ESCC which were collected from 2013 to 2015 by the research group,the expression level was divided into high expression group and low expression group according to the expression level.The relationship between RAB1 Aexpression and clinical characteristics and survival of patients with ESCC were analyzed,the effect of RAB1 Aoverexpression on the proliferation,migration and invasion ability of esophageal squamous cell carcinoma lines was detected.[Results]RAB1 Awas upregulated in ESCC cancer tissues than that in adjacent normal tissues(0.0113±0.0194 vs.0.0071±0.0098,P=0.0123),and its high expression was correlated with the family history of cancer(P=0.002),alcohol drinking(P=0.005),BMI(P=0.001),clinical stage(P=0.024),lymph node metastasis(P<0.001),distal metastasis(P<0.001),and pathologic type(P<0.001).The patients with a higher RAB1 Aexpression level had a shorter overall survival rate(P<0.001).Over expression of RAB1 Apromotes proliferation of ESCC cell lines(F=151.9,P<0.001),and promotes ESCC cell lines migration(100.4±14.2 vs.51.2±10.6,P=0.009)in trans-well assay,(655.0±43.9)μmvs.(320.7±115.2)μm,P=0.0003)in wound healing assay,and invasion(79.2±13.4 vs.46.2±17.6,P=0.0014).[Conclusion]RAB1 A was highly expressed in ESCC,higher expression of RAB1 A shorts the overall survival rate of ESCC patients.Upregulate the expression level of RAB1 Apromotes ESCC cell lines proliferation,migration and invasion.