不同生长调节剂配比对‘皖马铃薯1号’和‘皖马铃薯2号’试管苗生长的影响

Effect of different plant growth regulators on the growth of in vitro plantlets of Solanum tuberosum ’Wanmalingshu 1’ and ’Wanmalingshu 2’

‘皖马铃薯1号’和‘皖马铃薯2号’为安徽省农业科学院马铃薯研究团队选育,适宜安徽地区种植的早熟菜用马铃薯新品种。由于存在基因型差异,传统MS培养基所培养的试管苗较弱,不能满足后期进一步繁种的要求,为获得适宜两个新品种的壮苗培养基,以二者试管苗为试验材料,采用MS培养基添加不同比例α-萘乙酸(NAA)和6-苄氨基嘌呤(6-BA),进行不同生长调节剂配比对这两个马铃薯试管苗壮苗生长的影响试验,以筛选出适宜‘皖马铃薯1号’和‘皖马铃薯2号’的试管苗壮苗培养基。结果表明,‘皖马铃薯1号’试管苗最适壮苗培养基是MS+0.5 mg·L^-1 NAA+0.1 mg·L^-1 6-BA;‘皖马铃薯2号’最适壮苗培养基是MS+1.0 mg·L^-1 NAA+0.1 mg·L^-1 6-BA。

The new early-maturing potato varieties ‘Wanmalingshu 1’ and ‘Wanmalingshu 2’ were bred and selected by Institute of Horticulture, Anhui Academy of Agricultural Sciences. Due to the genetic background difference, in vitro plantlets cultured on traditional Murashige & Skoog (MS) medium were weak and could not meet further cultivation requirements. In order to obtain a suitable culture medium, in vitro potato plantlets of ‘Wanmalingshu 1’ and ‘Wanmalingshu 2’ were inoculated on MS medium supplemented with different concentrations of 1-Naphthylacetic acid (NAA) and 6-Benzylaminopurine (6-BA) to understand plant growth regulator effect on production of robust in vitro plantlets. The results showed that, the optimum medium for ‘Wanmalingshu 1’ is MS + 0.5 mg·L^-1 NAA + 0.1 mg·L^-1 6-BA, and the suitable medium for ‘Wanmalingshu 2’ is MS + 1.0 mg·L^-1 NAA + 0.1 mg·L^-1 6-BA.