超高效液相色谱法测定马齿苋提取物中羟基二氢博伏内酯

Hydroxydihydrobovolide from Portulaca Oleracea L. Extract Determination by Ultra-High Performance Liquid Chromatography Method

目的:超高效液相色谱法(UHPLC)法测定马齿苋提取物中羟基二氢博伏内酯含量。方法:采用Shimadzu Nexera X2 LC-30A超高效液相色谱仪进行检测,以乙腈-水(32∶68)为流动相;检测波长220 nm;流速1 mL/min;柱温40℃。结果:羟基二氢博伏内酯在8~48μg/mL浓度范围内与各峰面积呈良好的线性关系,回归方程为:Y=5 264.4X+1 427.2和相关系数r=0.999 3,平均加样回收率为98.03%,RSD为0.83%。精密度、重复性、稳定性良好。结论:本研究所建立的UHPLC分析方法简单,准确,重复性好,可用于马齿苋提取物中羟基二氢博伏内酯的含量测定,并为马齿苋提取物的质量控制提供了方法学参照依据。

Objective: The purpose is to determine the hydroxydihydrobovolide in the Portulaca oleracea L. extract. Method: The analysis was carried out on the Shimadzu Nexera X2 LC-30 A with the mobile of acetonitrile-water(32∶68), detection wavelength of 220 nm, flow rate at 1 mL/min, column temperature at 40 ℃. Result: The calibration curve was in good linearity over the range of 8~48 μg/mL, and the regression equation was Y=5 264.4 X+1 427.2(r=0.999 3). The average recovery was 98.03% and RSD of reproduction was 0.83%. Conclusion: The method was simple, accurate and reproducible, which was applied for the determination of hydroxydihydrobovolide in the Portulaca oleracea L. extract and can be used as the reference standard for the quality control of Portulaca oleracea L. extract.