天水106份冬小麦品种（系）Yr18基因和1BL/1RS易位的分子检测

Molecular Detection of Yr18 Gene and 1BL/1RS Translocation in 106 Winter Wheat Cultivars(Lines)in Tianshui

选用与Yr18基因和1BL/1RS易位系紧密连锁的SCAR和STS标记,对天水106份供试冬小麦育成品种(系)Yr18基因、1BL/1RS易位系的分布情况进行分子检测。结果表明,106份材料中,有2份材料检测到Yr18基因,有55份材料检测到1BL/1RS易位系,分别占被检测材料的1.89%、51.89%。对目前流行条锈菌小种有良好抗性且表现慢锈性的Yr18基因在甘肃天水小麦育种中的利用率较低,而1BL/1RS易位系的利用率仍然较高。品系15-16H307和07-144-2-1-1-1同时检测到1BL/1RS易位系和Yr18基因,建议在甘肃天水小麦抗条锈病育种中合理应用。

The distribution of Yr18 gene and 1BL/1RS translocation lines in 106 winter wheat cultivars(lines)in Tianshui were detected by using SCAR and STS markers closely linked to Yr18 gene and 1BL/1RS translocation line.The results showed that Yr18 gene was detected in 2 of 106 materials and 1BL/1RS translocation was detected in 55 materials,accounting for 1.89%and 51.89%of the tested materials respectively.The utilization rate of Yr18 gene with good resistance and slow rust resistance to the current epidemic stripe rust races in Tianshui wheat breeding in Gansu Province was low,while the utilization rate of 1BL/1RS translocation line was still high.The 1BL/1RS translocation line and Yr18 gene were detected in both strains 15-16H307 and 07-144-2-1-1 at the same time.It is suggested that they be reasonably applied in wheat stripe rust resistance breeding in Tianshui of Gansu Province.