胡麻SUC基因家族的鉴定与生物信息学分析

Identification and Bioinformatics Analysis of the SUC Gene Family in Flax

利用多个物种的SUC基因蛋白序列在胡麻基因组内进行BlastP分析,通过Pfam确认结构域,获得胡麻SUC基因家族成员,并进行基因结构分析;对蛋白分子量、等电点、信号肽、跨膜结构域、糖基化修饰位点、亚细胞定位、Motif及二级结构进行预测。结果鉴定得到12个胡麻SUC基因家族成员,大部分成员含有4个以上的外显子,10个以上跨膜结构域,并获4个特征性Motif。进化树分析显示,胡麻SUC分别属于SUT1、SUT2和SUT4家族。

In this study,multiple SUC protein sequences were analyzed in flax genome using BlastP,and the structural domain was confirmed by Pfam.The members of the SUC gene family were obtained.The analysis of gene structure,prediction of protein molecular weight,isoelectric point,signal peptide,transmembrane domain,glycation modification site,subcellular localization,Motif and secondary structure were carried out on the family menbers.The results showed that 12 members of flax SUC gene family were identified,containing 4 characteristic motifs,most of which had more than 4 exons,more than 10 transmembrane domains.The evolutionary tree analysis showed that flax SUC belonged to SUT1,SUT2 and SUT4 families,respectively.