金龙胶囊及其多糖、蛋白组分对人类脑胶质瘤小鼠原位模型的干预作用

Intervention effect of Jinlong capsule extract and its crude polysaccharides and proteins on mouse orthotopic implanted glioma

目的复方中药金龙胶囊(JLC)提取物及其制备的粗多糖、粗蛋白的抗脑肿瘤效果及安全性评价。方法运用外科原位接种(surgical orthotopic implantation)技术,将肿瘤块种植到裸小鼠颅内,建立小鼠原位模型。用FluorVivo Model 100荧光成像仪对所有小鼠的脑肿瘤进行非侵袭性的实时成像观察。通过对空白对照组(无肿瘤且溶剂为溶媒)、阴性对照组(溶媒)、阳性对照组(替莫唑胺32 mg/kg)、JLC提取物低(750 mg/kg)、中(1200 mg/kg)、高剂量组(1500 mg/kg)及粗多糖(100 mg/kg)、粗蛋白组(100 mg/kg)的肿瘤抑制率进行比较,评价经灌胃给予金龙胶囊提取物对小鼠原位模型肿瘤生长的影响。结果给药25 d后,JLC低、中剂量组肿瘤抑制率均为34.63%,与阴性对照组相比差异无显著性;粗多糖组和粗蛋白组肿瘤抑制率分别为22.26%、29.01%,肿瘤重量分别为(0.178±0.048)g、(0.144±0.047)g,与阴性对照组相比差异无显著性;阳性对照组和高剂量组,抑制率分别为99.57%、61.90%,肿瘤重量分别为(0.001±0.001)g、(0.088±0.045)g,差异有显著性(P<0.01,P<0.05)。结论本文研究结果表明,JLC粗多糖及粗蛋白有一定的脑胶质瘤抑制作用。肿瘤负荷和药物治疗可能为影响小鼠体重正常增长的因素;在现有剂量下,未发现明显的急性毒性反应(P>0.05)。

Objective To evaluate the antiglioma effect and safety of extracts of the Chinese medicine,Jinlong capsules(JLC),as well as the crude polysaccharides and proteins prepared from these extracts,to confirm the antiglioma efficacy and safety of the prepared JLC extracts.Methods One hundred 3-4-week old male BALB/c-nu nude mice were divided into the following groups:blank control(without tumor,solvent)negative control(solvent),positive control(temozolomide,32 mg/kg),low-dose JLC extract(750 mg/kg),medium-dose JLC extract(1200 mg/kg),high-dose JLC extract(1500 mg/kg),crude polysaccharides(100 mg/kg),and crude protein(100 mg/kg)groups.An orthotopic surgical technique was applied to implant a piece of human glioma U87-RFP tumor mass into the brains of nude mice to establish an orthotopic implantation model of U87-RFP glioma.After building the model,a FluorVivo Model 100 fluorescence imaging system was used for noninvasive real-time imaging of the brain tumors in the mice.The effects of JLC extracts on the growth of the orthotopic U87-RFP model were evaluated by comparing the tumor inhibition rates of these groups.Results After 25 days of intragastric administration of the drugs,the tumor inhibition rates of the low-dose and medium-dose groups were both 34.63%.and neither group’s weight differed significantly from that of the negative control group.For the crude polysaccharide and crude protein groups,the tumor inhibition rates were 22.26%and 29.01%,and the tumor weights were 0.178±0.048 g and 0.144±0.047 g,respectively,not significantly different from those of the negative control group.For the positive-control and high-dose groups,the tumor inhibition rates were 99.57%and 61.90%,and the tumor weights were 0.001±0.001 g and 0.088±0.045 g,respectively,which differed significantly(P<0.01 and P<0.05,respectively).Conclusions Our findings indicate that crude JLC polysaccharides and proteins have certain inhibiting effect on gliomas.At the doses used in this study,no obvious acute toxic effect is observed.