高脂饮食诱导肥胖对雌性生育大鼠卵巢功能的影响

Effect of obesity induced by a high-fat diet on ovarian function in female rats of reproductive age

目的评估高脂饮食诱导肥胖对雌性大鼠卵巢功能的影响,并探讨其作用机制。方法80只雌性Wistar大鼠随机分为高脂饮食(HFD)组(n=40)和正常饮食(对照)组(n=40)。饲养60d后评估各组大鼠发情周期及大鼠卵泡发育各个阶段细胞数量及凋亡情况,并检测各组大鼠血清孕酮和雌二醇浓度。然后按1∶2比例雄雌大鼠合笼3周。结果HDF诱导大鼠产生肥胖和高血糖,但是不影响大鼠血清甘油三酯、胆固醇和C-反应蛋白浓度。肥胖也改变卵巢功能,HFD大鼠发情间期延长,血清雌二醇浓度降低及窦闭锁卵泡数量增加。此外,HFD组大鼠也出现卵泡囊肿。而且HFD组大鼠怀孕晚于对照组大鼠。结论肥胖诱导大鼠出现卵泡囊肿,并干扰排卵,导致妊娠延迟。

Objective To evaluate whether high-fat diet-induced obesity affects ovarian function in rats and to contribute to an understanding of the mechanisms involved.Methods Female 5-week-old Wistar rats were fed a high-fat diet(HFD group,n=40)or a normal diet(control group,n=40).After 2 months of feeding,the estrous cycle and the number of cells in each stage of rat follicular development were evaluated.The levels of serum progesterone and estradiol in each group were also measured.Male and female rats(1∶2)were then mated over a period of 1 week.Results HFD induced obesity and hyperglycemia,without altering levels of serum triglycerides,cholesterol,or C-reactive protein.HFD also altered ovarian function by prolonging diestrous phases,decreasing serum estradiol concentrations,and increasing the number of antral atretic follicles.Moreover,the HFD group developed follicular cysts and became pregnant later than control group rats.Conclusions HFD induces follicular cyst development and disturbs the ovulatory process,leading to delayed pregnancy.