原发性痛风性关节炎患者miR-23a miR-24-2和miR-27a的表达水平及调节机制

The Expression Level and Regulation Mechanism of MicroRNA-23a MicroRNA-24-2 and MicroRNA-27a in Patients with Primary Gouty Arthritis

目的:分析原发性痛风性关节炎(PGA)患者microRNA-23a(miR-23a)、microRNA-24-2(miR-24-2)和microRNA-27a(miR-27a)的表达水平及调节机制。方法:将本院50例PGA患者为病例组,30例健康体检人员为对照组。比较两组实验室常规指标,通过实时荧光定量PCR法对miR-23a、miR-24-2和miR-27a的表达水平进行检测,并采用Pearson相关性分析miR-23a、miR-24-2、miR-27a表达间的关系,将所获数据纳入统计学分析。结果:病例组血浆空腹血糖、尿酸、白细胞计数、中性粒细胞、甘油三酯、载脂蛋白B100较对照组均明显升高(均P<0.05),载脂蛋白A1较对照组均明显下降(P<0.01);而两组总胆固醇的比较,并无明显差异(P>0.05)。相比对照组,病例组miR-23a、miR-24-2和miR-27a的表达水平明显下调(P<0.01)。经Pearson相关性分析结果显示,病例组患者外周血单个核细胞中miR-23a、miR-24-2、miR-27a三者之间的表达水平存在正相关关系(P<0.01)。采用尿酸盐(100μg/mL)对健康体检人员中的外周血单个核细胞进行刺激,结果发现在炎性微环境下,刺激组外周血单个核细胞中的miR-23a、miR-24-2和miR-27a的表达水平显著低于无刺激组(P<0.01)。结论:miR-23a、miR-24-2和miR-27a可能作为炎症负性调控因子而在PGA的炎症免疫反应过程中起到重要作用,并且miR-24-2可能具有调节PGA的脂蛋白的作用。

Objective: To analyze the expression level and regulation mechanism of microRNA-23a (miR-23a), microRNA-24-2 (miR-24-2) and microRNA-27a (miR-27a) in patients with primary gouty arthritis (PGA). Methods: 50 patients with PGA in our hospital were selected as case group and 30 health physical examination personnel as control group. The expression levels of miR-23a, miR-24-2 and miR-27a were detected by real-time fluorescent quantitative PCR. Pearson correlation analysis was used to analyze the relationship between the expressions of miR-23a, miR-24-2 and miR-27a. The data were included in statistical analysis. Results: The plasma fasting blood glucose, uric acid, white blood cell count, neutrophils, triglycerides and apolipoprotein B100 in the case group were significantly higher than those in the control group (all P<0.05), apolipoprotein A1 was significantly lower than those in the control group (P<0.01), but there was no significant difference in total cholesterol between the two groups (P>0.05). Compared with the control group, the expression levels of miR-23a, miR-24-2 and miR-27a in the case group were significantly decreased (P<0.01). Pearson correlation analysis showed that there was a positive correlation between the expression levels of miR-23a, miR-24-2 and miR-27a in peripheral blood mononuclear cells of patients (P<0.01). Urate (100 g/ml) was used to stimulate peripheral blood mononuclear cells in healthy people. The results showed that the expression levels of miR-23a, miR-24-2 and miR-27a in peripheral blood mononuclear cells in the stimulation group were significantly lower than those in the non-stimulation group (P<0.01). Conclusion: Mi-23a, mir-24-2 and mir-27a may play an important role in the inflammatory immune response of PGA as negative inflammatory regulators, and mir-24-2 may regulate the lipoprotein of PGA.