环状RNA ciRS-7和EGFR在子宫内膜癌组织中的表达水平

Expression Levels of Cyclic RNA ciRS-7 and EGFR in Endometrial Carcinoma

目的:探讨环状RNA ciRS-7和表皮生长因子受体(EGFR)在子宫内膜癌中的表达关系及临床意义.方法:收集2011年7月至2013年5月邯郸市第一医院收治的62例行手术治疗的EC患者肿瘤组织标本为研究组,同时收集60例因子宫肌瘤行子宫切除的正常子宫内膜组织作为对照组,采用实时荧光定量PCR技术(qRT-PCR)检测所有标本中ciRS-7、EGFR表达水平,分析二者表达水平相关性及其与临床病理特征和预后关系.结果:研究组组织中ciRS-7、EGFR表达水平明显高于对照组(P<0.05);ciRS-7表达与年龄、绝经情况无关(P>0.05),与组织学分化、病理分期、肌层浸润及淋巴结转移相关(P<0.05);EGFR表达与年龄无关(P>0.05),与绝经情况、组织学分化、病理分期、肌层浸润及淋巴结转移相关(P<0.05);相关性分析显示,EC患者组织中ciRS-7与EGFR表达呈正相关性(r=0.875,P<0.001);ciRS-7高表达组患者中位生存时间低于低表达组患者(P<0.05),EGFR高表达患者中位生存时间低于低表达组患者(P<0.05).结论:EC患者组织中ciRS-7、EGFR均高表达,二者呈正相关性,并与组织学分化、病理分期、肌层浸润、淋巴结转移及预后相关,推测二者均可作为EC预后评估的潜在生物标记物.

Objective: To investigate the expressions and clinical significances of cyclic RNA ciRS-7 and epidermal growth factor receptor (EGFR) in endometrial cancer. Methods: 62 patients tissus with endometrial cancer who underwent surgical treatment in Handan First Hospital from July 2011 to May 2013 were studied, meanwhile, 60 normal endometrial tissues of hysteromyoma patients undergoing hysterectomy were collected as control group, real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detected the expression levels of ciRS-7 and EGFR in all specimens, the correlation between the expressions of ciRS-7 and EGFR, and their relationships with clinicopathological characteristics and prognosis. Results: The expression levels of ciRS-7 and EGFR in the study group were significantly higher than those in the control group (P<0.05);the expression of ciRS-7 was not correlated with age and menopause (P>0.05), but with histological differentiation, pathological stage, myometrial invasion, and lymph node metastasis (P<0.05);the expression of EGFR was not related to age (P>0.05), but related to menopause, histological differentiation, pathological stage, myometrial invasion, and lymph node metastasis (P<0.05);the correlation analysis showed that there was a positive correlation between ciRS-7 and EGFR expression in endometrial cancer tissues (r = 0.875, P<0.001);the median survival time of patients with high expression of ciRS-7 was lower than that of patients with low expression (P<0.05), and the median survival time of patients with high expression of EGFR was lower than that of patients with low expression (P<0.05). Conclusion: The expressions of ciRS-7 and EGFR are high in endometrial cancer, they were positively correlated, and correlated with histological differentiation, pathological stage, myometrial invasion, lymph node metastasis, and prognosis, it is speculated that both of them can be used as potential biomarkers for prognostic evaluation of endometrial cancer.