血清miR-222和miR-206水平与女性阿尔茨海默病患者认知和运动功能的关系及其在诊断中的价值

Relationship between serum miR-222 and miR-206 levels and cognitive and motor function in female patients with Alzheimer’s disease and their value in diagnosis of Alzheimer’s disease

目的:探讨血清miR-222和miR-206水平与女性阿尔兹海默病(AD)患者认知和运动功能的关系及其在AD诊断中的价值。 方法:采用逆转录-聚合酶链反应方法检测66例AD女性患者(AD组)血清miR-222和miR-206相对表达量;应用简易精神状态检查(MMSE)、AD评定量表认知分量表(ADAS-Cog)、日常生活能力量表(ADL)、改良Bruce症状控制心电图平板运动实验评估AD患者的认知功能、生活能力及运动能力;结果与50名健康对照者(对照组)比较;分析血清miR-222和miR-206相对表达量与患者认知及运动功能的关系。结果:AD组血清miR-222相对表达量显著低于、miR-206相对表达量显著高于对照组(P均<0.01);AD组血清miR-222水平与MMSE评分、平板运动实验中最大代谢当量(METs)及最大心率呈正相关(r=0.412、0.509、0.423;P均<0.05),与ADAS-Cog评分、平板运动实验的心率-血压乘积(RPP)呈负相关(r=-0.531、-0.396;P均<0.05);AD组血清miR-206水平与MMSE评分、METs及最大心率呈负相关(r=-0.527、-0.557、-0.471;P均<0.05),与ADAS-Cog评分、RPP呈正相关(r=0.630、0.408;P<0.05或P<0.01);血清miR-222和miR-206诊断AD的ROC曲线下面积分别为0.703、0.784。 结论:女性AD患者血清miR-222、miR-206表达异常,并与其认知和运动能力下降相关;血清miR-222、miR-206表达水平对诊断AD具有一定临床价值。

Objective: To explore the relationship between serum miR-222 and miR-206 levels and cognition and motor function in female patients with Alzheimer's disease (AD) and their value in diagnosis of AD. Method: The relative expression of serum miR-222 and miR-206 in 66 female AD patients (AD group) were detected by reverse transcription-polymerase chain reaction.The mini mental status examination (MMSE), AD assessment scale cognitive subcale (ADAS-Cog),activities of daily living (ADL) and improved Bruce symptom control ECG treadmill exercise test were used to evaluate the cognitive function,life ability and exercise function;the results were compared with 50 healthy controls (control group).The relationship between the relative expression of serum miR-222 and miR-206 and the patient's cognitive and motor function were analyzed. Results: Compared with the control group,the relative expression of miR-222 was significantly lower and miR-206 was significantly higher in the AD group (all P <0.01).Serum miR-222 level in AD group was positive correlated with MMSE score,maximum metabolic equivalents(METs) and maximum heart rate ( r =0.412,0.509,0.423;all P <0.05),and was negative correlated with ADAS-Cog score and heart rate-blood pressure product (RPP)( r =-0.531,-0.396;all P <0.05).Serum miR-206 level in AD group was negatively correlated with MMSE score,METs,and maximum heart rate( r =-0.527,-0.557,-0.471;all P <0.05),and was positive correlation with ADAS-Cog score and RPP( r=0.630,0.408;P <0.05 or P <0.01).The areas under the ROC curve serum miR-222 and miR-206 levels were 0.703 and 0.784 for AD diagnosis. Conclusion: The expression of serum miR-222 and miR-206 in female AD patients are abnormal and related to their cognitive and motor functiou decline.Serum miR-222 and miR-206 expression levels have a certain clinical value in diagnosing AD.