基于改进爬上算法的数字显微镜自动对焦方法

Auto-focusing Method for Microscopy Based on Improved Hill-climbing Algorithm

自动对焦技术是计算机视觉、成像系统和各种精密仪器应用中的关键技术之一。随着科学技术的飞速发展,越来越多的人更加关心如何实现数字显微镜快速而精确的自动对焦问题。近年来人们对自动对焦技术的研究也多集中在数字图像处理的方法上。但是,在一般情况下,成像目标具有复杂性与不确定性的特点,因此,在数字成像系统中,现有的自动对焦技术仍然存在诸多不足,比如,对焦评价函数以及对焦的实时性与精确性等方面。通过对实验数据的分析,提出了一种改进的爬山算法通过在特定阶段采用特定对焦评价函数的方法,最终实现了自动对焦的目的,在保证调焦精度的情况下有效提高了自动对焦的速度。

Auto-focusing Technology is one of the key technologies in the application of computer vision,imaging systems and precision instruments.With the rapid development of science and technology,how to achieve fast and accurate autofocus in a digital imaging system is a problem which is getting more and more attention. In recent years,the research of the autofocus technology has been more concentrated in the digital image processing methods.However,due to the complexity and uncertainty of general imaging target,there are still many deficiencies in the existing autofocus technology.And there are still many problems focusing on the evaluation function and focus of real-time and accuracy.Around the characteristics of the microscopic imaging,numerous attempts and analysis on the experimental data have done,an improved hill-climbing algorithm is proposed.The improved hill-climbing algorithm use different focus evaluation function at different stages,and ultimately achieved the auto-focus,which efficiently improved the speed of the autofocus in the case of ensuring the precision of focusing.