海南岛5种按蚊种群对溴氰菊酯和DDT的敏感性测定及击倒抗性突变检测研究

SUSCEPTIBILITY OF FIVE ANOPHELES SPECIES TO DELTAMETHRIN AND DDT AND KNOCKDOWN RESISTANCE MUTATION DETECTION IN HAINAN ISLAND, CHINA

测定海南岛不同按蚊种群对杀虫剂溴氰菊酯和DDT的敏感性,分析击倒抗性的突变情况。结果显示,利用药膜接触筒法对采自海口市的5种按蚊种群进行测定,生物测定其对溴氰菊酯和DDT的敏感性,结合形态学及分子生物学方法鉴定种类,扩增并分析VGSC基因,检测1014位点的突变情况。海口的按蚊种群接触溴氰菊酯和DDT后的死亡率分别为72.73%和76.92%,均为抗性种群。从中华按蚊(n=5)、带足按蚊(n=7)、迷走按蚊(n=205)、棋斑按蚊(n=20)和浅色按蚊B(n=37)的kdr基因部分片段扩增和测序结果中,发现1014位点均无突变,为野生型。结果表明,海南岛5种按蚊种群对溴氰菊酯和DDT杀虫剂已产生抗药性,VGSC基因的1014位点突变不是其抗性产生的主要机制。

Aim of this study was to test the sensitivity of Anopheles population to deltamethrin and DDT, and analyze the mutation of knockdown resistance in Hainan Island, China. The standard tube bioassay was performed to test the susceptibility of collected samples to deltamethrin and DDT, morphological and molecular biology methods were used to identify the mosquito species. The VGSC gene fragments were amplified and analyzed for scanning the codon 1014 mutations. The mortality of Anopheles from Haikou were 72.73% and 76.92% to deltamethrin and DDT, respectively, showed they were the resistant populations. Partial fragments of kdr gene from 274 Anopheles mosquitoes were identified including An. sinensis(n=5), An. peditaeniatus(n=7), An. vagus(n=205), An. tessellatus(n=20) and An. subpictus B(n=37). Sequencing of their VGSC gene showed that codon 1014 was wild type and no mutations were detected. The Anopheles population in Hainan Island has developed resistance to deltamethrin and DDT. The L1014 mutation of VGSC gene should not be the main resistances mechanism.