血清CXCL16、CCK-18在胆石症患者中的表达水平及临床意义

Expression and clinical significance of serum CXCL16 and CCK-18 in patients with cholelithiasis

目的探讨血清CXC趋化因子配体16(CXCL16)、胆囊收缩素-18(CCK-18)在胆石症患者中的表达水平及临床意义。方法选取西安医学院附属医院收治的63例胆石症患者作为研究对象,另选取40例同期健康体检者作为对照组。采用酶联免疫吸附法检测单纯性胆石症组患者与复杂性胆石症组患者的血清CXCL16、CCK-18表达水平。采用Logistic回归方法分析影响单纯性胆石症进展为复杂性胆石症的相关因素。采用受试者工作特征曲线(ROC)分析CXCL16、CCK-18诊断复杂性胆石症的效能。结果胆石症患者的血清CXCL16及CCK-18表达水平明显高于对照组,复杂性胆石症组患者的血清CXCL16、CCK-18表达水平明显高于单纯性胆石症组,差异均有统计学意义(P均<0.05)。单因素及多因素Logistic回归分析显示,肝硬化、腹腔积液、低密度脂蛋白胆固醇(LDL-C)、CXCL16、CCK-8与单纯性胆石症进展为复杂性胆石症密切相关。ROC曲线分析显示单指标诊断复杂性胆石症时,CXCL16诊断的ROC曲线下面积(AUC)大于CCK-18,CXCL16联合CCK-18诊断复杂性胆石症的AUC为0.955,明显大于单指标检测。结论血清CXCL16及CCK-18在胆石症患者中表达水平升高。血清CXCL16及CCK-18表达水平与复杂性胆石症相关,两者联合检测可辅助诊断复杂性胆石症。

Objective This paper intends to investigate the expression and significance of serum CXC chemokine ligand 16(CXCL16)and cholecystokinin-18(CCK-18)in patients with cholelithiasis.Methods Sixty-three cases of cholelithiasis in Xi′an NO.1 Hospital were selected as the research object while forty healthy persons in the same period were selected as the control group.The expressions of serum CXCL16 and CCK-18 in patients with simple cholelithiasis and complex cholelithiasis were detected by enzyme-linked immunosorbent assay.Logistic regression analysis was used to analyze the related factors affecting the progression of simple cholelithiasis to complex cholelithiasis.The value of CXCL16 and CCK-18 in the diagnosis of complex cholelithiasis was analyzed by ROC.Results The expressions of serum CXCL16 and CCK-18 in patients with cholelithiasis were significantly higher than those in the control group(P<0.05)while the expressions of serum of CXCL16 aand CCK-18 in patients with complex cholelithiasis were significantly higher than those in patients with simple cholelithiasis,and the difference was statistically significant(P<0.05).Univariate and multivariate logistic regression analysis showed that cirrhosis,peritoneal effusion,LDL-C,CXCL16,and CCK-8 were closely related to the progression of simple cholelithiasis to complex cholelithiasis.The ROC curve analysis showed that the AUC of CXCL16 was higher than that of CCK-18 in the diagnosis of complex cholelithiasis,and the AUC of CXCL16 combined with CCK-18 was 0.955,which was significantly higher than that of CXCL16 and CCK-18 alone in the diagnosis of complex cholelithiasis.Conclusions The expression of serum of CXCL16 and CCK-18 increased in patients with cholelithiasis.CXCL16 and CCK-18 are associated with complex cholelithiasis,the combined detection of which can assist in the diagnosis of complex cholelithiasis.