高选择性和高灵敏度的Fe-MOFs催化生色传感研究

A Fe-MOFs-based catalytic sensing system with high selectivity and sensitivity

将催化反应引入光谱传感体系中可大大提高其灵敏度,因此,开展催化生荧/催化生色传感研究具有重要意义.将组成固定并具有孔隙结构的金属有机框架材料(MOFs)用于催化光谱传感研究,有助于理解催化剂与传感性能之间的关系.然而,目前涉及此方面研究的报道相当有限.本文采用水热合成法制备了一种比表面积和空隙率大、催化活性高的Fe-MOFs材料(Fe-MIL-101).以3,3′,5,5′-四甲基联苯胺(TMB)为底物分子,在H2O2的存在下,系统地研究了该Fe-MOFs材料催化TMB生色反应的实验条件以及其催化反应机理.在此基础上,借助谷胱甘肽(GSH)与其他巯基化合物在清除·OH自由基能力方面的显著性差异,建立了一种高效、高选择性、高灵敏度的GSH催化光谱传感体系.这种高选择性明显优于以往的GSH光谱传感与识别体系.

Since introducing catalytic reactions can greatly improve the sensitivity of spectral sensing systems,developing catalytic chromogenic/fluorescence sensing systems has important influence. By introducing metal-organic frameworks(MOFs) with fixed composition and good porous structure into traditional spectral sensing, it is helpful for understanding the relationship between catalyst and its sensing performance. However, very limited examples involved in this field at present. In this article, Fe-MOFs(Fe-MIL-101) with large surface area and high porosity were successfully synthesized by using a hydrothermal synthesis method. The Fe-MOF could exhibit outstanding catalytic activity towards the traditional enzyme substrate 3,3′,5,5′-tetramethylbenzidine(TMB). The experimental conditions and catalytic reactions mechanism of Fe-MOF catalyzing the oxidation reaction of the TMB-H2O2 system were systematically studied. On the basis of these findings, and taking together the fact that glutathione(GSH) serving as a better free radical scavenger than other traditional thiols, a highly efficient GSH sensing system with high selectivity and sensitivity could be successfully established.