摘要
目的:评估前列腺癌(PCa)组织中P504s、34β-E12、P63表达情况,并探讨PCa多模态MRI参数与P504s的相关性。方法:对43例PCa患者和64例非PCa患者(均经前列腺穿刺活检或根治性前列腺切除术后病理证实)的多模态MRI影像资料(常规MRI、扩散加权成像DWI、动态增强磁共振成像DCE-MRI)进行回顾性分析,获取时间-信号强度(SI-T)曲线,计算Tmax(s)、SImax%、Rmax%和自动生成ADC值;运用免疫组化方法检测前列腺组织中P504s、34β-E12、P63表达情况。结果:PCa组P504s、34β-E12、P63阳性表达率分别为83.7%、25.6%、25.6%,非PCa组分别为0%、91.0%、86.0%,两组间差异有统计学意义(P<0.05);PCa组和非PCa组ADC值、Tmax(s)、SImax%、Rmax%分别为:(0.83±0.22)×10^-3mm^2/s、(21.30±10.78)s、(1.75±0.39)%、(20.20±15.50)%和(1.34±0.28)×10^-3mm^2/s、(50.22±36.31)s、(1.24±0.41)%、(7.98±6.25)%,组间比较差异有统计学意义(P<0.05);ADC值、Tmax(s)与P504s表达呈负相关(r=-0.60、-0.37,P<0.01);而SImax%、Rmax%与P504s表达呈正相关(r=0.50、0.45,P<0.01)。结论:PCa多模态MRI各参数与P504s表达有相关性,可作为预测肿瘤恶性程度的影像生物学标志物。
Objective: To evaluate the expressions of P504s, 34β-E12, and P63 proteins in PCa and the correlation between the parameters of multi-modality MRI and the expression of P504s. Methods: We retrospectively analyzed the multi-modality MRI data on 43 PCa and 64 non-PCa patients. We obtained the signal intensity-time(SI-T) curves, maximum SI(SImax), time to SImax(Tmax), rate of maximum enhancement(Rmax), and automatically generated apparent diffusion coefficient(ADC) values by conventional, diffusion-weighted and dynamic contrast-enhanced MRI, and determined the expressions of P504s, 34β-E12 and P63 in the prostatic tissues of the patients by immunohistochemistry. Results: Statistically significant differences were observed between the PCa and non-PCa groups in the positive expressions of P504s(83.7% vs 0%, P < 0.05), 34β-E12(25.6% vs 91.0%, P < 0.05) and P63(25.6% vs 86.0%, P < 0.05) in the prostatic tissue, the ADC value([0.83 ± 0.22] vs [1.34 ± 0.28]×10^-3mm^2/s, P < 0.05), Tmax([21.30 ± 10.78] vs [50.22 ± 36.31] s, P < 0.05), SImax([1.75 ± 0.39]% vs [1.24 ± 0.41]%, P < 0.05), and Rmax([20.20 ± 15.50]% vs [7.98 ± 6.25]%, P < 0.05). The expression of P504s was correlated negatively with the ADC value and Tmax(r =-0.60 and-0.37, P < 0.01) but positively with SImax and Rmax(r = 0.50 and 0.45, P < 0.01). Conclusion: The parameters of multi-modality MRI are correlated with the expression of P504s in PCa and can be used as imaging biomarkers for predicting the degrees of its malignancy.
作者
张家伟
刘佳佳
黄婷
赵莹莹
蔡磊
陈志强
ZHANG Jia-wei;LIU Jia-jia;HUANG Ting;ZHAO Ying-ying;CAI Lei;CHEN Zhi-qiang(School of Clinical Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China;Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China)
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2019年第7期619-625,共7页
National Journal of Andrology
基金
宁夏自然科学基金(NZ13280,NZ1234)
宁夏医科大学国内一流建设学科临床医学资助项目(NXYLXK2017A05)
宁夏回族自治区重点研发计划项目(2019BEG03033)~~