红树林植物内生真菌Stachybotrys chartarum HDN16-358次级代谢产物研究

Study on the secondary metabolites of a mangrove endophytic fungus Stachybotrys chartarum HDN16-358

目的对红树林植物内生真菌Stachybotrys chartarum(HDN16-358)进行次级代谢产物的研究。方法利用薄层色谱层析(TLC)、反相ODS、Sephadex LH-20、半制备型HPLC等色谱学方法对菌株的乙酸乙酯粗提物进行分离纯化;利用NMR、MS、UV等波谱学技术手段,解析确定化合物的结构,并对化合物1进行了细胞毒活性筛选。结果分离鉴定了5个单体化合物:(2S)-5-Hydroxy-2,7-dimethyl-2-(4,8-dimethyl-3E,7-nonadienyl)-2H-chromene-6-carbaldehyde(1)、BR-011 (2)、LL-Z 1272β(3)、stachybotrylactone (4)、5-Methylbenzene-1,3-diol(5)。结论红树林植物内生真菌是活性物质的重要来源。从红树林植物内生真菌Stachybotrys chartarum HDN16-358中分离得化合物1~5,其中化合物1首次从自然界中分离得到,其具有中等强度的细胞毒活性。

Objective To investigate the secondary metabolites of a mangrove endophytic fungus Stachybotrys chartarum HDN16-358.Methods The EtOAc extract of fungus HDN 16-358 was isolated and purified by TLC,ODS,Sephadex LH-20,semi-prepararative HPLC,etc.The structures of these compounds were identified based on spectroscopic analysis(NMR,MS,CD,etc.)as well as comparison with the related literature.Compound 1 was tested in vitro for its cytotoxicity activity.Results Five compounds were obtained and identified as(2 S)-5-Hydroxy-2,7-dimethyl-2-(4,8-dimethyl-3 E,7-nonadienyl)-2 H-chromene-6-carbaldehyde(1),BR-011(2),LL-Z 1272β(3)and stachybotrylactone(4),5-Methylbenzene-1,3-diol(5).Conclusion Mangrove endophytic fungi were important microbial resources of bioactive constituents.Compounds 1~5 were isolated from mangrove endophytic fungus Stachybotrys chartarum HDN16-358.Notably,compound 1 was isolated from nature for the first time,and displayed moderate cytotoxicity activity.