铜绿假单胞菌褐藻胶合成抑制剂筛选模型的建立

Establishment of screening models for the inhibition of alginate synthesis in Pseudomonas aeruginosa

目的建立以铜绿假单胞菌(Pseudomonas aeruginosa)乙酰化褐藻胶的合成为靶向的筛选模型,用于筛选抑制铜绿假单胞菌褐藻胶合成的活性分子。方法分别建立铜绿假单胞菌双层平板筛选模型和PalgDGFP荧光分子模型,从海洋微生物代谢产物中筛选抑制铜绿假单胞菌乙酰化褐藻胶合成的活性分子。结果利用2个筛选模型筛选300余种化合物,获得2种抑制铜绿假单胞菌合成褐藻胶的化合物,且化合物的抑制作用具有浓度依赖性,验证了模型的可行性和有效性。结论 2种模型结合,可以高效、准确地筛选目标化合物,为解决细菌耐药问题提供了1种有效的手段。

Objective To establish a few models for discovering compounds which could inhibit the synthesis of acetylated alginate in Pseudomonas aeruginosa.Methods The Pseudomonas aeruginosa doublelayer plate screening model and the PalgD-GFP fluorescence model were established.Compounds isolated from marine microorganisms were screened based on the above models.Results After verification of the feasibility and effectiveness,the models were used to screen over 300 compounds.In consequence,two compounds inhibiting the synthesis of acetylated alginate were obtained,and the inhibition of the compounds were concentration-dependent.Conclusion The joint use of the two models could help screening the target compounds efficiently and provide a feasible and effective method for solving bacterial resistance.