系统性硬化症患者血清中可溶性人类白细胞抗原-G的表达及与Th17细胞相关性的研究

Expression of serum sHLA-G and correlation with Th17 cell in serum of patients with systemic sclerosis

目的检测系统性硬化症(SSc)患者血清可溶性人类白细胞抗原-G(s HLA-G)水平,分析其与Th17细胞的关系,探讨血清s HLA-G在系统性硬化症发病机制中的可能作用。方法酶联免疫吸附法(ELISA)检测40例SSc患者和40例健康体检者血清s HLA-G水平及Th17细胞代表因子IL-17水平。结果 SSc患者血清s HLA-G水平为(24. 85±6. 21) U/ml,低于健康体检者的(36. 33±6. 74) U/ml;IL-17在SSc中水平为(30. 65±10. 06) pg/ml,明显高于健康体检者的(9.79±2.97) pg/ml,上述差异均有统计学意义(P <0. 01);s HLA-G与IL-17呈明显负相关(r=-0. 879,P <0. 01)。结论 s HLA-G作为免疫调节分子,因其在SSc中的低表达,使SSc免疫紊乱,促进免疫细胞向Th17分化,从而加速了疾病的发生发展。

Objective To detect the level of serum soluble human leukocyte antigen-G( s HLA-G) in patients with systemic sclerosis( SSc),analyze its relationship with Th17 cells,and explore the possible role of serum s HLA-G in the pathogenesis of systemic sclerosis. Methods The serum concentrations of s HLA-G and IL-17 production in SSc patients and healthy controls were measured with enzyme-linked immunosorbent assay( ELISA). Results Serum s HLA-G levels in SSc patients was( 24. 85 ± 6. 21) U/ml,which was lower than in healthy controls [( 36. 33 ± 6. 74) U/ml],and IL-17 levels in SSc was( 30. 65 ± 10. 06) pg/ml,significantly higher than the control group [( 9. 79 ± 2. 97) pg/ml],and the above differences were statistically significant( P < 0. 01);sHLA-G and IL-17 showed a significant negative correlation( r =-0. 879,P < 0. 01).Conclusion As an immunomodulatory molecule,the low expression of sHLA-G in SSc causes SSc immune disorder and promotes the differentiation of immune cells into Th17,thereby accelerating the development of the disease.