液相色谱串联质谱法测定水果中多菌灵残留量

Determination of Carbendazim Residues in Fruits by LC-MS/MS

为建立一种测定苹果、猕猴桃、脐橙等水果中多菌灵残留量的液相色谱串联质谱检测方法,在弱碱性条件下采用乙酸乙酯振荡提取, MCX固相萃取柱净化,用液相色谱串联质谱仪测定,外标法定量。结果表明,多菌灵在5.0~200 ng/mL范围内呈良好的线性关系,线性相关系数为0.999 9;在10.0, 20.0和40.0μg/kg浓度水平下,添加回收率范围为88.2%~100.5%,相对标准偏差RSD (n=6)为2.05%~8.78%,方法定量限为0.01 mg/kg。

A method was developed for the determination of carbendazim residues in fruits such as apple, kiwifruit and navel orange by liquid chromatography-tandem mass spectrometry(LC-MS/MS). Under weak alkaline conditions, the sample was extracted with ethyl acetate and purified by MCX column, determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS) and quantified by external standard method. The results showed that carbendazim had a good linear relationship in the range of 5.0 ng/mL to 200 ng/mL, the linear correlation coefficient was 0.999 9, the recovery ranged from 88.2% to100.5% at the levels of 10.0, 20.0 and 40.0 μg/kg, and the relative standard deviation RSD of precision was 2.05% to 8.78%(n=6). The quantitative limit of the method was 0.01 mg/kg.