鱼鳞胶原基复合抗菌海绵制备工艺优化及性能研究

Preparation process optimization and properties of fish scale collagenbased antibacterial sponge

以鱼鳞胶原蛋白为基材,加入壳聚糖复合改性,并添加丁香油为抗菌剂,甘油为增塑剂,采用冷冻干燥法制备胶原蛋白/壳聚糖复合抗菌海绵衬垫,研究胶原蛋白与壳聚糖比例、成海绵溶液浓度、甘油含量、丁香油含量对复合海绵包装性能的影响,在单因素试验基础上进行正交试验,综合正交试验极差分析与方差分析优化制备复合抗菌海绵制备工艺。结果表明:胶原蛋白复合抗菌海绵衬垫的优化制备工艺条件为:胶原与壳聚糖比例6:4、成海绵溶液浓度2%、甘油含量10%、丁香油含量1%。此条件下,复合海绵抗张强度413.72kPa、断裂伸长率19.03%、吸水倍数32.15、溶失率10.96%、抑菌圈面积(大肠杆菌)234.54mm^2、抑菌圈面积(金黄色葡萄球菌)349.19mm^2。

Cross-linking fish scale collagen and chitosan,adding clove oil as an antibacterial agent,and glycerin as a plasticizer to make a collagen/chitosan composite antibacterial sponge by freeze-drying.The effects of collagen and chitosan ratio,solution concentration,glycerin content and clove oil content on the properties of composite sponges were studied.Through orthogonal experiments,the optimum process conditions of composite antibacterial sponge were determined by range analysis and variance analysis.The results showed that the optimal preparation conditions of collagen composite antibacterial sponge liner were as follows:collagen/chitosan ratio of 6:4,solution concentration of 2%,glycerin content of 10%,and clove oil content of 1%.The properties of the composite antibacterial sponge liner prepared under these conditions were tensile strength of 413.72 kPa,elongation at break of 19.03%,water absorption multiple of 32.15,dissolution rate of 10.96%,inhibition zone area(Escherichia coli)of 234.54 mm^2,and inhibition zone area(Staphylococcus aureus)349.19 mm^2.