HS-SPME-GC-MS法分析不同辐照方式的人参酒挥发性成分

Analysis of volatile components of ginseng liquor by different irradiation methods by HS-SPME-GC-MS method

试验采用顶空固相微萃取(HS-SPME)的提取方法对人参酒中挥发性成分进行气相色谱-质谱(GC-MS)分析,以面积归一法计算各成分的相对含量。分别在原人参酒、电子束辐照酒、60Co-γ辐照酒中鉴定出25、30、16种挥发性物质,其中萜烯类化合物是人参酒的主要挥发性成分,相对含量分别为80.39%、55.87%、80.29%。人参酒共同挥发性成分有8种,其中马兜铃烯、α-古芸烯、β-人参烯是主要成分,辐照前后人参酒挥发性成分的种类和含量均发生明显变化,并各自形成了独有挥发性成分。

In this experiment,the volatile components in ginseng liquor were analyzed by gas chromatography-mass spectrometry(GC-MS)by the extraction method of headspace-solid phase microextraction(HS-SPME),the relative content of each component was calculated by area attribution method.25,30 and 16 volatile substances were identified in the original ginseng liquor,electron beam irradiation liquor and 60Co-γirradiation liquor.Terpene compounds were the main volatile components of ginseng liquor,the relative content is 80.39%,55.87%,80.29%,respectively.There were 8 kinds of common volatile components of ginseng liquor,of which aristolene,α-gurjunene andβ-Panasinsene were the main components,the types and contents of volatile components of ginseng liquor before and after irradiation changed significantly,and each formed a unique volatile component.