羊种布氏杆菌介导巨噬细胞mmu-miR-146a-5p的差异表达及其靶基因的初步鉴定

Differential expression of mmu-miR-146a-5p in macrophages mediated by Brucella melitensis and preliminary identification of its target genes

羊种布氏杆菌(Brucella melitensis,B.melitensis)感染巨噬细胞后,运用高通量测序技术挖掘出mmu-miR-146a-5p差异表达。利用TargetScan和miRDB数据库分别进行mmu-miR-146a-5p靶基因的在线预测,结果分别有224和125个,利用韦恩分析发现,2个数据库的在线预测结果交集有70个;进一步利用PicTar数据库进行mmumiR-146a-5p靶基因的在线预测,并与韦恩分析结果取交集;转染mmu-miR-146a-5p mimics至巨噬细胞中,通过荧光定量PCR方法检测预测靶基因的相对表达量,发现Ptpra与Tfdp2表达量显著降低,结果初步表明,mmu-miR-146a-5p的靶基因为Ptpra与Tfdp2。该试验为进一步研究mmu-miR-146a-5p在羊种布氏杆菌感染巨噬细胞过程中的作用奠定了基础。

After Brucella melitensis (B.melitensis)infected with macrophages,the differential expression of mmu-miR-146a-5p was detected by high throughput sequencing technology.The TargetScan and miRDB database were used to predict the mmu-miR-146a-5p target gene online,respectively. The results were 224and 125,respectively.Using wayne analysis,there were 70online prediction results of two databases,and the PicTar database was further used to predict the mmumiR- 146a-5p target,and the results were analyzed with the results of waysis.mmu-miR-146a-5p mimics were used to transfect the macrophages,the relative expression of the target gene was detected by the fluorescence quantitative PCR method,and the expression of Ptpra and Tfdp2was significantly reduced.The result preliminarily showed that the target of mmu-miR-146a-5p was due to Ptpra and Tfdp2.This experiment will pave the way for further study on the role of mmumiR- 146a-5p in the process of B.melitensis infection macrophages.