含伯氏疏螺旋体OspA、OspC蛋白VLPs的构建及鉴定

Construction and identification of VLPs containing Borrelia burgdorferi OspA and OspC proteins

莱姆病是一种经蜱传播的人兽共患病,随着人们经济的发展,伴侣动物的不断出现增加了人与动物经蜱传播莱姆病的风险,而当前并未有治疗莱姆病的有效方法,因此研制莱姆病疫苗成为一大热点。为了更好地预防莱姆病的发生,本研究通过替换新城疫病毒血凝素-神经氨酸酶蛋白(haemagglutinin-neuraminidase protein,HN)胞外域的方式,利用昆虫杆状病毒表达系统分别构建了含伯氏疏螺旋体候选抗原表位外膜蛋白A(outer surface protein A,OspA)、外膜蛋白C(outer surface protein C,OspC)的新城疫病毒样颗粒(virus-like particles,VLPs),形成了含有伯氏疏螺旋体抗原表位的新城疫病毒样颗粒,为之后研制莱姆病疫苗提供了新思路、新想法。

Lyme disease,caused by the bacterium Borrelia burgdorferi,is a zoonotic disease through the bite of infected ticks.Companion animals have always suffered from tick infestations and then increased the risk of humans infected with the bacterium Borrelia burgdorferi through biting of ticks from itselives.However,there is no effective methods for Lyme disease treatments. Therefore,in current study,we developed two novel Newcastle disease virus-like particles (VLPs)vaccine candidates co-expressing either outer surface protein A (OspA)or outer surface protein C(OspC)of Borrelia burgdorferi via replacing the extracellular domain of the Newcastle disease virus hemagglutinin-neuraminidase protein(HN).Taken together,our current results indicated that the Newcastle disease virus-like particle vaccine expressing the candidate epitopes of Borrelia burgdorferi provides new ideas for the development of Lyme disease vaccines.