玉米赤霉烯酮通过干扰内分泌系统造成性成熟雄性大鼠生殖损伤

Zearalenone causes reproductive damage in mature male rats by disrupting the endocrine system

玉米赤霉烯酮(ZEA),作为一种环境类雌激素物,其对动物及人体健康具有潜在的危害。尤其是,ZEA可能会导致人类不孕不育症。本试验中,给予性成熟的雄性SD大鼠每公斤单次腹腔注射5mg/kg的ZEA,在随后的48h内观察检测。可见大鼠血清睾酮(T)和促黄体生成素(LH)在6h极显著降低,而血清LH在随后12h又极其显著升高;睾丸环腺苷酸(cAMP)在6h同样显著下降;同时,睾丸LHR,Star,p450scc,3β-hsd-1mRNA表达水平在注射ZEA后6h显著下调。总之,本研究可以得出单次腹腔注射ZEA可能通过扰乱内分泌系统,影响胆固醇合成中关键酶的基因表达,进而抑制睾酮生成,最终造成生殖系统短暂性的损伤。

Zearalenone(ZEA),as an environmental estrogen,is potential harmful to animal and human health.Especially,ZEA may lead to infertility in humans.In the study,mature male SD rats were given a single intraperitoneal injection of 5mg/kg of ZEA for observing 48h.The study result showed that the levels of testosterone(T)and luteinizing hormone(LH)in serum were extremely significantly reduced at 6h,while the levels of serum LH were extremely significantly increased at 12h.cAMP also decreased significantly at 6h.At the same time,the mRNA expression of testicular LHR,Star,p450scc,3β-hsd-1was significantly downregulated at 6hafter the injection of ZEA.In short,the study showed that a single intraperitoneal injection of ZEA disturbed the endocrine system by interfering with the secretion of LH in the body and affected the gene expression of key enzymes in cholesterol synthesis.Thus,it hindered the production of testosterone and eventually caused temporary damage to the reproductive system.