柴胡皂甘d与阿奇霉素对人型支原体活性的对比研究

The comparative study on the activity of saikosaponin d and azithromycin against Mycoplasma hominis

目的比较柴胡皂昔d(Sd)与阿奇霉素对人型支原体在体外的抗菌活性。方法采用液体微量稀释法,对于临床分离出的20株人型支原体(Mh),分别测定Sd以及阿奇霉素对20株Mh的最低抑菌浓度。采用配对f检验,确定二者的抗菌活性是否有差异。结果Sd对20株Mh的最底抑菌浓度(MIC)在0.5~16mg/L,MIC50为1mg/L;阿奇霉素对20株Mh的MIC范围在0.5~128mg/L,MIC50为2mg/L。结论Mh对阿奇霉素的耐药率比较普遍,Sd对Mh在体外有较好的抑菌作用,二者抗菌活性差异有统计学意义。

Objective To study the antibacterial activity of saikosaponin D ( Sd ) and azithromycin against Mycoplasma hominis in vitro. Methods The minimal inhibitory concentration of saikosaponin D and azithromycin to 20 strains of Mycoplasma hominis were determined by liquid microdilution method for 20 strains of Mycoplasma hominis isolated from clinic. Paired t test was used to determine whether the antibacterial activity of the two groups was statistically significant. Results The MIC range of saikosaponin D to 20 strains of Mycoplasma hominis was between 0.5 to 16mg/L and MIC50 was lmg/L. The MIC range of azithromycin to 20 Mycoplasma hominis was between 0.5 and 128mg/L and MIC50 was 2mg/L. Conclusion The resistance rate of Mycoplasma hominis to azithromycin is more common, and there is a good difference in bacteriostasis in vitro, and the difference of the antibacteria activity of the two is statistically significant.