空气中丙烯醛与丙酮检测方法的优化

The Optimization for Determining Acrolein and Acetone in Air

采用高效液相色谱仪(HPLC)方法对我国发布的HJ 683—2014《环境空气醛、酮类化合物的测定高校液相色谱法》13种醛酮进行定量检测时,普通色谱柱难以彻底分离丙烯醛与丙酮。通过采用Acclaim Carbonyl C18色谱柱,对柱温、流速、流动相、流动相配比等色谱柱条件的优化,使丙烯醛与丙酮在较短的时间内得到有效的分离。在柱温25℃,流速1.5 m L/min,流动相乙腈与甲醇的体积比为1∶1时,分离度为2.59,且13种醛酮类标准物质全部分析完成时间在45 min内,能够很好的消除丙酮与丙烯醛在定量检测中的叠加影响。为环境空气的监测提供了一种更加快速、准确、科学的分析方法。

A new method was optimized for determining 13 kinds of different volatile aldehydes and ketones in ‘Ambient air-Determination of aldehyde and ketone compounds-High performance liquid chromatography’(HJ 683-2014) by high performance liquid chromatography(HPLC). Parameters of the chromatography condition were optimized, such as column oven temperature, flow rate, mobile phase and mobile phase ratio. The results showed that acrolein and acetone were separated effectively in a short time with acclaim carbonyl C18 column. The temperature of column oven was 25 ℃, the flow rate was 1.5 mL/min, and elution solvent was a mixture of acrolein and acetone(1 : 1). In these chromatography conditions, the separation degree of acrolein and acetone was 2.59 and the analysis time of 13 aldehydes and ketones reference materials was 45 minutes, the superposition effect of acetone and acrolein quantitative monitoring could be eliminated well. The developed method is more rapid, accurate, scientific. It is suitable for monitoring of 13 aldehydes and ketones in the air.