两种植物甾醇纳米粒的降胆固醇效果研究

Cholesterol-lowering Effect of Two Types of Phytosterol Nanoparticles

目的:研究两种植物甾醇纳米粒对高血脂小鼠的降胆固醇效果。方法:建立高血脂小鼠模型,随后将小鼠分为8组:正常组、高脂模型组、阳性对照组、植物甾醇(PS)组、植物甾醇纳米粒(PNP)组、空白纳米粒(NP)组、壳聚糖修饰植物甾醇纳米粒(CS-PNP)组、空白壳聚糖修饰纳米粒(CS-NP)组,连续干预4周。测定小鼠体重、采食量变化,脏器指数、血清指标、肝脂含量以及粪便中甾醇含量,并进行肝脏切片观察。结果:各给药组对小鼠采食量及体重无显著影响(p> 0.05);PNP和CS-PNP组小鼠肝系数较高脂组显著降低(p <0.05),心系数、脾系数显著升高(p <0.05),对肺系数和肾系数无显著影响(p> 0.05);PNP和CS-PNP组可显著降低小鼠血脂中TG、TC和LDL-C水平,升高HDL-C水平,同时显著降低肝脏中TG与TC含量(p <0.05);PNP和CS-PNP组能有效治疗小鼠的脂肪肝。结论:PNP和CSPNP对膳食诱导的高血脂小鼠有较好的降胆固醇效果。

Objective: To investigate the cholesterol-lowering effect of two types of phytosterol nanoparticles on hyperlipidemic mice.Methods: The mice were divided into 8 groups randomly after the successful establishment of the high-fat diet( HFD)-induced hyperlipidemia model: normal group,hyperlipidemic model group,positive control group,phytosterol( PS) group,phytosterol nanoparticles( PNP) group,nanoparticles without phytosterol( NP) group,chitosan-modified phytosterol nanoparticles( CS-PNP) group,and chitosan-modified nanoparticles without phytosterol( CS-NP) group. The intervention of different samples continued for 4 weeks.The cholesterol-lowering effect of two types of phytosterol nanoparticles was determined by analyzing the average body weight and the daily food intake of the mice,the serum biochemical indices,the organ indices,the hepatic fat contents,the sterol contents in the feces and histological observation of liver slices. Results: The PNP and CS-PNP had no significant effect on the average body weight and the daily food intake of mice( p > 0.05).The hepatic indexes of hyperlipidemia mice in the PNP and CS-PNP groups were significantly decreased and the cardiac and splenic indexes were markedly increasing compared to the hyperlipidemic model group( p < 0.05).There had no significant difference in the pulmonary and nephritic indexes( p > 0.05).The PNP and CS-PNP dramatically decreased the TG,TC,and LDL-C levels,and increased the HDL-C level in serum( p < 0.05). The PNP and CS-PNP were the most potent in reducing the TG and TC contents in the mice liver( p < 0.05).The histopathological observation on the liver of the mice showed that PNP and CS-PNP were the most potent to reduce hepatic steatosis in hyperlipidemia mice( p < 0.05).Conclusion: The PNP and CS-PNP markedly reduced HFD-induced dyslipidemia,hypercholesterolemia and hepatic steatosis in mice.