NDRVσC蛋白互作的宿主因子筛选及生物信息学分析

IDENTIFICATION AND BIOINFORMATICS ANALYSIS OF HOST CELLULAR PROTEINS INTERACTING WITH NDRV σC PROTEIN

研究鸭呼肠孤病毒(Duck reovirus,DRV)σC蛋白与宿主因子之间的相互作用,对揭示σC蛋白在病毒复制过程中的功能及该病毒的致病机理具有重要作用。本研究采用免疫共沉淀法及质谱分析技术,筛选出与σC蛋白可能发生互作的宿主相关蛋白,并对所筛选出的宿主细胞蛋白进行了GO注释和KEGG代谢通路注释。结果表明,与空白对照组比较,获得与σC蛋白相互作用的宿主细胞蛋白有100个;筛选出的这些宿主细胞蛋白中,在病毒感染后不同时间点差异表达量均上升3倍以上的蛋白有6个,分别与病原致病性、蛋白质代谢途径、细胞信号通路转导有关。该研究结果为进一步探讨σC蛋白的生物学功能奠定了基础,也为揭示σC蛋白与宿主因子相互作用进而调控DRV复制等机制研究提供线索。

Exploring the interaction between σC protein of duck reovirus and host cellular proteins is important for revealing its function in viral replication.In this study,co-immunoprecipitation was employed to screen host-associated proteins that might interact with σC protein.The results were analyzed by GO annotation and KEGG pathway annotation.As compared with the control group,51 host cellular proteins were identified to be involved in this process by bioinformatics analysis,of which 6 proteins expressed more than 3 times.These host cellular proteins were closely related to protein metabolism,transduction of cell signaling pathways and pathogenicity of pathogens.The results indicated that the findings of the present study provided a foundation for elucidating the associated molecular mechanisms of the interaction of host cellular proteins with viral proteins in regulating the viral replication and pathogenesis.