2013—2017年我国小反刍兽疫病毒H基因分子演化特征

Molecular Evolution Characteristics of H Gene of Peste Des Petits Ruminants Virus in China During 2013 to 2017

为研究我国小反刍兽疫病毒(PPRV)的分子流行特点,对2013—2017年我国37株PPRV流行毒株进行血凝蛋白(H)基因序列测定和生物信息学分析。结果显示:37个毒株H基因核苷酸序列之间的遗传距离为0~0.0077,变异分布在41个位点,H蛋白氨基酸序列之间的遗传距离为0~0.0132,变异分布在24个位点。与15株代表毒株进行序列比对发现,2013-2017年我国37株PPRV流行毒株H基因的13个位点发生了核苷酸序列突变,其中9个导致了氨基酸序列的改变。以最大似然法构建分子进化树,发现2013-2017年我国流行的37个毒株构成基因4系中一个独立的进化小分支。本研究阐明了2013-2017年我国PPRVH基因的分子演化特征,从而为该病控制和消灭策略的制定提供了数据支持。

In order to study molecular characteristics of peste des petits ruminants virus(PPRV)in China, 37 prevalent strains of PPRV collected from 2013 to 2017 were used for hemagglutinin(H)gene sequencing and bioinformatics analysis. The results showed that the genetic distance among nucleotide sequences of H gene of these strains was 0 to 0.007 7,involving 41 variable sites,and the genetic distance among amino acid sequences of H protein ranged from 0 to 0.013 2,with 24 variable sites. Based on the sequence alignment against 15 reference strains, it was found that the mutation of nucleotide sequences took place in 13 variable sites of H gene of all these strains,of which,9 nucleotide mutations resulted in the change of amino acid sequence. In addition,a molecular evolutionary tree was constructed by maximum likelihood,it was found that the 37 studied strains could be grouped into a distinct clade in lineage IV. In short,the molecular evolution characteristics of H gene of PPRV in China from 2013 to 2017 were stated in this paper,which would provide data supports to formulate relevant strategies for control and eradication of the disease.