新疆某猪场母猪繁殖障碍病病原学检测和毒株鉴定

Pathogen Detection of Sow Reproductive Disorder Disease and Identification of Relevant Strain in a Swine Farm in Xinjiang

2018年4月,新疆某规模化繁育猪场出现大量母猪流产,产死胎、木乃伊胎等繁育障碍症状。为确诊病因,采集胎儿组织和母猪流产分泌物,通过普通PCR、RT-PCR、real-timePCR技术,对猪繁殖与呼吸综合征病毒(PRRSV)、伪狂犬病病毒(PRV)、猪细小病毒(PPV)、弓形虫4种病原进行检测。结果显示,PRRSV检测呈阳性,PRV、PPV和弓形虫检测呈阴性。通过PRRSV特异性引物NSP2进行PRRSV毒株鉴定,确定该猪场存在PRRSV类NADC30毒株。结果表明,类NADC30毒株是导致该猪场出现母猪繁殖障碍的重要病原,说明该新型毒株已经蔓延到新疆地区,并开始对该地生猪养殖业造成危害。本试验为新疆地区猪场母猪繁殖障碍的病因调查提供了依据。

In April 2018,some symptoms of reproductive disorders,such as abortion,stillbirth and mummified fetus,appeared in a large number of sows in a scaled breeding farm in Xinjiang. In order to identify all related causes,some fetal tissues and abortive secretions from sows were sampled to detect porcine reproductive and respiratory syndrome virus(PRRSV),pseudorabies virus(PRV),porcine parvovirus(PPV)and Toxoplasma gondii through normal PCR,RT-PCR and real time PCR technology respectively. The results showed that the samples were positive for PRRSV,and negative for PRV,PPV and Toxoplasma gondii. The NADC30-like strain of PRRSV was identified in the farm by NSP2,the specific primer of PRRSV. Besides,the reproductive disorders in the farm may be mainly caused by the NADC30-like strain,indicating that the new strain had been spreading to Xinjiang and begun to bring great harm to local pig-production industry. In view of that,some references were hereby provided for investigating the causes of sow reproductive disorders in Xinjiang.