姜黄素对宫内发育迟缓断奶仔猪肠道抗氧化功能的影响

Effect of Curcumin Supplementation on Intestinal Antioxidant Function in Weaning Piglets with Intrauterine Growth Retardation

目的:探讨姜黄素对宫内发育迟缓(intrauterine growth retardation,IUGR)断奶仔猪肠道抗氧化功能的影响。方法:选取16头正常初生体质量(normal birth mass,NBM)仔猪和16头IUGR仔猪,公母各半。26日龄断奶,分别饲喂基础日粮或姜黄素日粮(基础日粮+400 mg/kg姜黄素),分为N组(NBM仔猪+基础日粮)、NC组(NBM仔猪+姜黄素日粮)、I组(IUGR仔猪+基础日粮)和IC组(IUGR仔猪+姜黄素日粮),每组8头。饲养至50日龄屠宰取样,进行抗氧化指标的检测。结果:1)日粮中添加姜黄素后,与I组相比,IC组仔猪空肠蛋白质羰基(protein carbonyl,PC)、8-羟化脱氧鸟苷的含量和回肠丙二醛、PC的含量显著降低(P<0.05);2)与I组相比,IC组仔猪空肠总抗氧化能力、过氧化氢酶(catalase,CAT)活力显著升高(P<0.05),谷胱甘肽含量显著增多(P<0.05);回肠CAT活力显著升高(P<0.05),过氧化氢(H2O2)含量显著降低(P<0.05);3)与I组相比,IC组仔猪空肠核因子E2相关因子2(nuclear factor-erythroid 2-related factor 2,Nrf2)、CAT、超氧化物歧化酶、谷胱甘肽硫转移酶(glutathione S-transferase,GST)和NAD(P)H:醌氧化还原酶(NAD(P)H:quinone oxidoreductase l,NQO1)基因的表达显著升高(P<0.05);回肠Nrf2、GST、NQO1和硫氧还蛋白还原酶基因的表达显著升高(P<0.05)。结论:向日粮中添加400 mg/kg姜黄素对缓解IUGR诱导的仔猪肠道氧化应激、提高肠道抗氧化功能有一定作用。

Purpose: To investigate the protective effect of curcumin on intestinal oxidative damage in weaning piglets with intrauterine growth retardation(IUGR). Methods: A total of 16 newborn piglets with normal birth mass(NBM) and 16 newborn piglets with IUGR, half male and half female were selected for this study. After weaning at the age of 26 days,these piglets, fed on a basal diet(diet 1) or the same diet supplemented with 400 mg/kg curcumin(diet 2), were allocated to 4 groups of 8 animals each: NBM + diet 1(group N), NBM + diet 2(group NC), IUGR + diet 1(group I), and IUGR +diet 2(group IC). Every piglets were fed to 50 d, slaughtered, sampled, and then tested for antioxidant index. Results:Compared with group I, group IC significantly reduced protein carbonyl(PC) and 8-hydroxy-2’-deoxyguanosine contents in jejunum tissue, and malondialdehyde and PC contents in ileum tissue(P < 0.05). Compared with group I, group IC showed a significant increase in total antioxidant capacity(T-AOC), catalase(CAT) activity and glutathione(GSH) content in jejunum tissue(P < 0.05);a significant increase in CAT activity and a significant decrease in H2O2 content in ileum tissue(P < 0.05).Compared with group I, the mRNA expression levels of nuclear factor-erythroid 2-related factor 2(Nrf2), CAT, superoxide dismutase(SOD), glutathione S-transferase(GST) and NAD(P)H : quinone oxidoreductase l(NQO1) were significantly increased in jejunum tissue of group IC(P < 0.05), and the mRNA expression levels of Nrf2, GST, NQO1 and thioredoxin reductase 1 were significantly increased in ileum tissue(P < 0.05). Conclusion: Dietary addition of curcumin at a dose of400 mg/kg can alleviate intestinal oxidative stress caused by IUGR and improve intestinal antioxidant status.