霉菌检测中封闭式培养方法的建立和应用

Establishment and Application of a Closed Culture Method for Enumeration of Molds

通过微氧胁迫下黑曲霉的生长动态研究,构建霉菌的封闭式培养方法,并用于食品中霉菌的检测,对比分析霉菌封闭式培养方法的可行性和实用性。结果表明,建立的封闭式培养方法与传统敞开式培养方法的计数结果无显著差异。因此,对于食品中霉菌计数,可以采用以空气与培养基的体积比不小于3为参数的封闭式培养方法,代替传统的敞开式培养方法,以解决霉菌孢子扩散和污染的难题。

In the present study, we focused on the growth dynamics of Aspergillus niger under-micro oxygen stress to develop a closed culture method for the detection of this mold in foods. A comparative analysis was made to explore the feasibility and practicality of the culture method. The results showed that there was no significant difference between the closed culture and the traditional open culture. Therefore, the closed culture method featuring the parameter of volume ratio between air and medium equal to or more than 3 could be used to determine mold counts in foods in place of the traditional open culture method, so as to overcome the problems of mold spore diffusion and pollution.