链霉菌KIB-H1556次级代谢产物抗植物真菌活性研究

Metabolites against plant pathogenic fungi from Streptomyces sp.KIB-H1556

对曼陀罗(Datura stramonium L.)根际链霉菌Streptomyces sp.KIB-H1556的次级代谢产物进行研究,利用硅胶柱色谱、凝胶柱色谱和半制备HPLC等分离手段对其发酵产物进行分离纯化,采用MS和NMR等波谱学手段并结合文献数据鉴定了3个单体化合物的结构,分别为:Bafilomycin D(1)、Bafilomycin B1(2)和Bafilomycin B2(3)。初步抗植物病原真菌活性筛选发现化合物2和3具有广谱抗真菌活性,尤其对玉米病原真菌的抑制活性显著,可作为玉米病原真菌病害的潜在生物防治剂。

The secondary metabolites of Streptomyces sp.KIB-H1556 that an actinomycetes isolated from the rhizosphere soil of Datura stramonium L.were investigated.The extracts were purified by silica gel column,Sephadex LH-20 chromatography and semi-preparative HPLC.The structures of compounds were elucidated by NMR and mass spectrometry,and comparison with the reported data.Three compounds were identified as Bafilomycin D (1 ),Bafilomycin B1 (2 ) and Bafilomycin B2 (3 ).The antifungal assays demonstrated that compounds2 and3 possessed broad-spectrum antifungal activities against pathogenic fungi,especially pathogenic fungi related to corn.This result suggests that bafilomycins can been developed as potential antifungal agent for corn.