云南柑橘黄化脉明病毒的田间样品检测

Detection of CitrusYellowVeinClearingVirus(CYVCV)in Yunnan Province

柑橘黄化脉明病毒(Citrus yellow vein clearing virus,CYVCV)引起的黄脉病是威胁我国柑橘产业发展的一种重要病毒性病害.云南是我国的柑橘产区之一,为明确CYVCV在云南田间的感染情况,本研究于云南省瑞丽市、怒江州、凤仪镇和丽江市4地共采集33份疑似柑橘黄脉病样品,对其进行了PCR检测.结果显示,在33份田间疑似病样中有10份样品检测到CYVCV,总检出率为30.3%.统计表明,在不同寄主样品中CYVCV的检出率差异较大;柠檬样品CYVCV检出率最高,为50%;而香橼样品和香橙上检出率为0.各地区样品中CYVCV的检出率有一定差异性;其中瑞丽样品检出率最高,达43.5%;而怒江、凤仪及丽江样品中均未检测出CYVCV.

Yellow vein clearing disease,caused by citrus yellow vein clearing virus (CYVCV),is an important virus disease threatening the development of citrus industry in China.Yunnan is one of the citrus producing areas in China.In order to clarify the occurrence of CYVCV in Yunnan orchards,we collected 33 suspected citrus yellow vein disease samples from Ruili,Nujiang,Fengyi and Lijiang of Yunnan Province and detected them by PCR.The results showed that CYVCV was detected in 10 of the 33 suspected samples,the total detection rate being 30.3%.Statistical results showed that the detection rate of CYVCV in different host samples varied greatly.The detection rate of CYVCV in lemon [ Citrus limon (L.) Burm.f.] samples was the highest (50%),while that in citron ( C.medica ) and orange ( Citrus spp.) samples was 0%.The detection rate of CYVCV in different regions was different.Among them,Ruili had the highest detection rate (43.5%),while no CYVCV was detected in samples from Nujiang,Fengyi and Lijiang.