腺苷对大鼠股动脉狭窄远端A2b受体表达与血清NO浓度的影响

Effects of adenosine on A2b receptor and nitric oxide in a rat model of femoral artery stenosis

目的探讨腺苷对股动脉狭窄远端腺苷A2b受体表达与血清一氧化氮(NO)浓度的影响。方法取40只 Wistar 大鼠,对其中20只大鼠进行结扎股动脉术制备狭窄模型后,随机分为模型生理盐水组(A组)、模型腺苷组(B组),剩余20只大鼠只进行股动静脉分离,并随机分为对照生理盐水组(C组)和对照腺苷组(D组)。采用免疫组化法检测股动脉远端腺苷A2b受体的表达,硝酸还原酶法检测血清中NO的含量。结果免疫组化结果,B组的腺苷A2b受体阳性率低于D组( P <0.05),且D组高于A、C两组。硝酸还原酶法结果,B组NO在血清中含量远低于D组( P <0.01),且D组高于A、C两组。结论腺苷可致股动脉狭窄远端腺苷A2b受体表达和NO的浓度明显降低。

Objective To investigate the effects of adenosine on the expression of adenosine A2b receptor and serum nitric oxide (NO) concentration in femoral artery stenosis. Methods The femoral artery stenosis model were induced in 20 of 40 Wistar rats, which were randomly divided into model saline group (A), model adenosine group (B).The stenosis model was made by femoral artery ligation except for the control group (control saline group (C) and control adenosine group (D).The rats in the control groups were treated with woring line under the femoral artery without ligation.All of them were injected with saline or adenosine in the tail vein. The expression level of adenosine A2b receptor in quadriceps femoris was detected with immunohistochemistry and the concentration of NO in serum was measured with nitrate reductase method. Results Immunohistochemistry: The positive rate of adenosine A2b receptor in group B was far lower than that in group D, the results of group D was higher than that in group A and C ( P <0.05). Nitrate reductase results showed: The serum NO concentration in group B was lower than that in group D ( P <0.01), and the group D was higher than group A and group C. Conclusion Adenosine can significantly reduce A2b receptor expression and NO concentration in the distal end of femoral artery stenosis.