银屑病小鼠焦虑样行为学检测与分析

Detection and Analysis Anxiety-like Behavior in Psoriasis Mice

目的通过旷场实验以及洞板实验评估银屑病对小鼠焦虑样行为学的影响。方法10个月龄的雄性小鼠20只,随机分为2组,对照组和模型组各10只。通过咪喹莫特诱导银屑病小鼠模型。1周后应用旷场实验以及洞板实验方法,检测小鼠焦虑样行为学改变。结果与对照组相比,银屑病模型组小鼠其探索潜伏期较正常对照组延长,但无统计学意义。旷场实验中,银屑病模型组总路程、平均速度、中央区域停留时间、中央区域探索次数较对照组减少,洞板实验中,银屑病小鼠模型组探洞次数较正常对照组减少。结论银屑病可导致小鼠出现焦虑样行为。

Objective In order to compare the results on anxiety of the psoriasis in mice using open field test and the holeboard test.Methods Twenty C57BL/6J mice were randomized equally into blank control group (fed with normal forage and treated with vaseline) and model group (fed with normal forage and treated with 5% imiquimod cream).The open field test and the hole -board test were used to study the anxiety in the psoriasis mice.Results There was no difference in exploratory latency.Compared with the control group,the total distance of movement,average velocity,residence time in the central grid and the times of exploratory were increased in the psoriasis group.Moreover,the psoriasis mice show less hole exploration in the hole board test.Conclusion Psoriasis can induce the anxiety behavioral in mice.