EV71和COXa16感染手足口病患儿血清IL-6及IL-37水平检测的临床意义

Clinical significance of detection of serum IL-6 and IL-37 levels in children with hand, foot and mouth disease infected with EV71 and COXa16

目的检测EV71和COXa16感染手足口病(HFMD)患儿血清白细胞介素-6(IL-6)和白细胞介素-37(IL-37)水平并分析其意义。方法选取2012年1月1日~2017年12月31日我院儿科收治175例HFMD患儿作为研究对象,其中确诊的单纯EV71感染患儿100例,单纯CoxA16感染患儿65例,EV71和CoxA16合并感染患儿10例,另选取同期50例健康体检儿童作为对照组,酶联免疫吸附测定(ELISA)法检测血清IL-6和IL-37水平并分析其关系。结果EV71组、CoxA16组及EV71合并CoxA16组的IL-6、IL-37水平均显著高于对照组,差异有统计学意义(P<0.05);EV71组的IL-6、IL-37水平均显著高于CoxA16组、EV71合并CoxA16组,差异有统计学意义(P<0.05)。EV71组重症患儿的IL-6、IL-37水平均显著高于轻症患儿,差异有统计学意义(P<0.05)。EV71组IL-6与IL-37水平成正相关(P<0.05);EV71组IL-6与外周血单核细胞绝对值成正相关(P<0.05);EV71组IL-37水平与外周血中性粒细胞绝对值成正相关(P<0.05)。结论IL-6和IL-37参与了EV71和COXa16感染所致HFMD发病的病理生理过程,并与疾病的严重程度有关。

Objective To detect the serum levels of interleukin-6(IL-6)and interleukin-37(IL-37)in children with hand,foot,and mouth disease(HFMD)caused by EV71 and COXa16 infections and to analyze their clinical significance.Methods A total of 175 children with HFMD admitted to pediatrics department of our hospital from January 1,2012 to December 31,2017 were collected as subjects of study,and among of them 100 children were diagnosed with EV71 infection,65 children with CoxA16 infection,10 children with EV71 and CoxA16 co-infection.Another 50 healthy children at the same period were selected as the control group.Enzyme linked immunosorbent assay(ELISA)was used to detect the serum levels of IL-6 and IL-37 and their relationship was analyzed.Results The levels of IL-6 and IL-37 in EV71 group,CoxA16 group and EV71 combined with CoxA16 group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).The levels of IL-6 and IL-37 in EV71 group were significantly higher than those in CoxA16 group and EV71 combined with CoxA16 group,and the differences were statistically significant(P<0.05).The levels of IL-6 and IL-37 of severe children in EV71 group were significantly higher than those in mild children,and the differences were statistically significant(P<0.05).There was a positive correlation between IL-6 and IL-37 levels in EV71 group(P<0.05).The level of IL-6 in EV71 group was positively correlated with the absolute value of peripheral blood mononuclear cells(P<0.05).The level of IL-37 in EV71 group was positively correlated with the absolute value of peripheral blood neutrophils(P<0.05).Conclusion The IL-6 and IL-37 are involved in the pathogenesis of HFMD caused by EV71 and CoxA16 infections,and related to the severity of the disease.